Distinct actions of benzene and its metabolites on nitric oxide production by bone marrow leukocytes.

Benzene is a widely used industrial solvent known to cause bone marrow depression. This is associated with increased production of reactive oxygen metabolites and nitric oxide by bone marrow phagocytes, which have been implicated in hematotoxicity. Benzene metabolism to phenolic intermediates appears to be an important factor in bone marrow toxicity.

Simplified PCR-based detection and typing strategy for human papillomaviruses utilizing a single oligonucleotide primer set.

Utilizing the PCR, we have devised a detection and typing system for the human papillomaviruses (HPVs) that are commonly associated with preneoplastic and cancerous lesions of the genital and aero-digestive tracts: HPV 6, 11, 16 and 18. Utilizing computer sequence analysis, we designed a single, "consensus" set of oligomeric nucleotide primers capable of amplifying a 571-594-bp region of the E1 open reading frame of all of these HPVs. Detection via PCR amplification is followed by restriction endonuclease digestion of the resultant products that yield distinctive and reproducible banding patterns by polyacrylamide gel electrophoresis because of their internal sequence diversity.

Rubeola.

Although immunization practices have dramatically reduced the incidence of measles, several populations remain at risk of the disease. Modified measles may occur in those who received immune globulin in childhood. Atypical measles, often confused with Rocky Mountain spotted fever, may develop in those who received inactivated measles virus vaccine and were subsequently exposed to the natural disease.

Monoclonal antibodies to the glycoprotein of vesicular stomatitis virus: comparative neutralizing activity.

Nineteen independently isolated hybridomas producing monoclonal antibodies to the glycoprotein of vesicular stomatitis virus were isolated and studied for their capacity to neutralize viral infectivity. By measuring competitive binding of 125I-labeled monoclonal antibodies in a radioimmunoassay. 11 different, non-cross-reacting antigenic determinants were identified on the vesicular stomatitis virus G protein.