Chemistry, manufacturing and controls strategies for using novel excipients in lipid nanoparticles.

Lipid nanoparticles (LNPs) for nucleic acid delivery often use novel lipids as functional excipients to modulate the biodistribution, pharmacokinetics, pharmacodynamics and efficacy of the nucleic acid. Novel excipients used in pharmaceutical products are subject to heightened regulatory scrutiny and often require data packages comparable to an active pharmaceutical ingredient. Although these regulatory requirements may help to ensure patient safety they also create economic and procedural barriers that can disincentivize innovation and delay clinical investigation.

The role of automation etiquette and task-criticality on performance, workload, automation reliance, and user confidence.

Previous research suggests good automation etiquette can yield positive effects on user performance, trust, automation reliance, and user confidence - especially in personified or anthropomorphized technologies. The current study examined the impact of automation etiquette and task-criticality in non-personified technology. The study used a computer-based automation task to examine good and bad automation etiquette models and different domain-based task-criticality levels (between-subjects) that contained various stages of automation (stage 2 and stage 3) and automation reliability levels (60% and 80%) (within-subjects).

Polite AI mitigates user susceptibility to AI hallucinations.

With their increased capability, AI-based chatbots have become increasingly popular tools to help users answer complex queries. However, these chatbots may hallucinate, or generate incorrect but very plausible-sounding information, more frequently than previously thought. Thus, it is crucial to examine strategies to mitigate human susceptibility to hallucinated output.

Chemical mapping of the surface interactome of PIEZO1 identifies CADM1 as a modulator of channel inactivation.

The propeller-shaped blades of the PIEZO1 and PIEZO2 ion channels partition into the plasma membrane and respond to indentation or stretching of the lipid bilayer, thus converting mechanical forces into signals that can be interpreted by cells, in the form of calcium flux and changes in membrane potential. While PIEZO channels participate in diverse physiological processes, from sensing the shear stress of blood flow in the vasculature to detecting touch through mechanoreceptors in the skin, the molecular details that enable these mechanosensors to tune their responses over a vast dynamic range of forces remain largely uncharacterized. To survey the molecular landscape surrounding PIEZO channels at the cell surface, we employed a mass spectrometry-based proteomic approach to capture and identify extracellularly exposed proteins in the vicinity of PIEZO1.