Publications by authors named "R C Ordonez"
Female sexual behaviors in rodents (lordosis and appetitive or "proceptive" behaviors) are induced through a genomic mechanism by the sequential actions of estradiol (E2) and progesterone (P), or E2 and testosterone (T) at their respective receptors. However, non-steroidal agents, such as gonadotropin-releasing hormone (GnRH), Prostaglandin E2 (PGE2), noradrenaline, dopamine, oxytocin, α-melanocyte stimulating hormone, nitric oxide, leptin, apelin, and others, facilitate different aspects of female sexual behavior through their cellular and intracellular effects at the membrane and genomic levels in ovariectomized rats primed with E2. These neurotransmitters often act as intermediaries of E2 and P (or T).
View Article and Find Full Text PDFThe conventional way steroid hormones work through receptors inside cells is widely acknowledged. There are unanswered questions about what happens to the hormone in the end and why there isn't always a strong connection between how much tissue takes up and its biological effects through receptor binding. Steroid hormones can also have non-traditional effects that happen quickly but don't involve entering the cell.
View Article and Find Full Text PDFArticle Synopsis
- The text discusses the creation of gmctool, an online tool designed to predict metabolic weaknesses in cancer cells, which is important for systems biology research.
- This tool utilizes a concept called genetic Minimal Cut Sets (gMCSs) to analyze genome-scale metabolic networks and includes a database of synthetic lethals derived from the latest metabolic map of human cells.
- Notably, gmctool has shown improved performance over earlier algorithms and has been applied to multiple myeloma, a type of blood cancer, providing experimental evidence for the critical roles of specific enzymes in certain patient groups.
Article Synopsis
- - The study investigates how genomic context affects gene regulation, focusing on the Igf2/H19 locus in mice, where CTCF binds to a control region that determines which gene is activated based on enhancers.
- - By using synthetic regulatory genomics to replace the native genetic locus with 157-kb payloads, researchers discovered new long-range regulatory relationships and how enhancers interact with their environment.
- - The research found that while the H19 enhancers depend on their native location, the Sox2 locus control region operates independently, suggesting that the context of enhancers is crucial for their function across different cell types.