Characterization of DNA methylation reader proteins in .

In plants, cytosine DNA methylation (mC) is largely associated with transcriptional repression of transposable elements, but it can also be found in the body of expressed genes, referred to as gene body methylation (gbM). gbM is correlated with ubiquitously expressed genes; however, its function, or absence thereof, is highly debated. The different outputs that mC can have raise questions as to how it is interpreted-or read-differently in these sequence and genomic contexts.

The Building Respectful Integrated Doula Support as a Gateway for Enhanced Maternal Health Outcomes and Experiences Conceptual Framework for Integrating Doula Support in the Pregnancy Continuum.

Doulas, non-clinical companions trained to provide emotional, informational, and physical support throughout the pregnancy continuum, have emerged as cost-effective interventions to enhance maternal health. This article aims to review existing definitions, roles, outcomes, and theoretic frameworks surrounding doula support, culminating in the development of the Building Respectful Integrated Doula Support as a Gateway for Enhanced Maternal Health Outcomes and Experiences (BRIDGE) conceptual framework. The BRIDGE conceptual framework provides a comprehensive understanding of doula support and its potential to improve maternal health outcomes, underscoring the importance of integrating doula support into standard maternal health care practices.

Establishment of single-cell transcriptional states during seed germination.

Germination involves highly dynamic transcriptional programs as the cells of seeds reactivate and express the functions necessary for establishment in the environment. Individual cell types have distinct roles within the embryo, so must therefore have cell type-specific gene expression and gene regulatory networks. We can better understand how the functions of different cell types are established and contribute to the embryo by determining how cell type-specific transcription begins and changes through germination.

Early moderate prenatal alcohol exposure and maternal diet impact offspring DNA methylation across species.

Alcohol consumption in pregnancy can affect genome regulation in the developing offspring but results have been contradictory. We employed a physiologically relevant murine model of short-term moderate prenatal alcohol exposure (PAE) resembling common patterns of alcohol consumption in pregnancy in humans. Early moderate PAE was sufficient to affect site-specific DNA methylation in newborn pups without altering behavioural outcomes in adult littermates.