Four novel nontuberculous mycobacteria species: sp. nov., sp. nov., sp. nov. and sp. nov. revived from a historical Danish strain collection.

Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S , internal transcribed spacer and 23S elements, as well as a single-copy core-gene (, , and ) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains.

Family caregiver involvement in forensic mental health care - A qualitative study of healthcare professionals' perceptions.

This qualitative study aims to explore healthcare professionals' (HCPs) perceptions of facilitators and barriers in their collaboration with family caregivers in forensic mental health care (FMHC). A thematic analysis resulted in two interrelated themes: (1): Organizational constraints, with subthemes Resource-driven time and staffing constraints, Duty of confidentiality, and External substance use treatment, and (2) Collaboration with family caregivers, with subthemes Alliance in collaboration, and Overinvolvement. The findings show that HCPs prefer family caregivers to support care and treatment, reflecting a unilateral direction of support.

Development of a PCR assay for rapid and accurate detection of an emerging clone in the Capital Region of Denmark.

Objectives: To develop and validate a real-time PCR assay detecting the sequence bridging Tn and the chromosome in the emerging vancomycin-resistant (VREfm) clone (ST80/CT2406).

Methods: The Tn insertion site was determined on routinely sequenced VREfm isolates. The outer boundaries of Tn and adjoining host bacterial sequences were determined using a BLAST search in the silent information regulator gene .

Face and content validity of the EMPOWER-UP questionnaire: a generic measure of empowerment in relational decision-making and problem-solving.

Background: Decision-making and problem-solving processes are powerful activities occurring daily across all healthcare settings. Their empowering potential is seldom fully exploited, and they may even be perceived as disempowering. We developed the EMPOWER-UP questionnaire to enable assessment of healthcare users' perception of empowerment across health conditions, healthcare settings, and healthcare providers' professional backgrounds.