Effect of cyclophosphamide on spleen lymphocytes in mice.

The experiments were carried out in the following groups of Swiss mice: 1. after administration of a single dose of cyclophosphamide (300 mg/kg); 2. after i.

Effect of cyclophosphamide on the thymus in mice.

A histologic and morphometric evaluation of weight of the thymus and content of Fc and C3 lymphocytes after a single dose of 300 mg cyclophosphamide per kg body weight in mice of the Swiss strain revealed a drop in the weight of the thymus and morphologic changes, manifested by elimination of lymphocytes from the cortical part of the thymus, followed by return to normal of the weight of the thymus and at the same time a rise in percentages of Fc and C3 receptor lymphocytes.

The influence of cyclophosphamide on lymph node and peripheral blood lymphocytes in mice.

The research was carried out on mice of the Swiss strain in the following groups: after a single dose od cyclophosphamide (CY--300 mg/kg), after the administration of sheep erythrocytes (2 X 10(8) into the pads of hind feet and on animals sheep erythrocytes were given to on the fourth days after the CY administration. On the days following the administration of CY, a histological evaluation of the popliteal lymph nodes was performed. The total number of peripheral blood lymphocytes and the number of EA and EAC-rosette forming lymphocytes in lymph nodes and peripheral blood was established.

Proliferation of human lymphocytes by mitogen stimulated cells.

Lymphocytes cultured for three days with PHA or Con A were capable of inducing vigorous proliferation of autologous or allogeneic lymphocytes. Kinetics of the response varied with mitogen dose used for obtaining stimulating cells and with the ratio of stimulating to responder cells. A requirement for viable, mitogen-reactive lymphocytes cultured for at least two days with the mitogen, for their contact with the reacting cells and an absence of significant quantities of mitogen on Con A lymphoblasts examined by electron microscopy demonstrated that a mitogen itself was not responsible for the stimulation.