Publications by authors named "R Bosotti"
Article Synopsis
- - Human CD4EOMES T cells consist of diverse types, including Th1-cells, Tr1-cells, and CD4CTL, each with different functions in the immune response.
- - Tr1-cells are anti-inflammatory while non-classical EOMES Th1-cells are pro-inflammatory, but both share features like producing IFN-γ and granzyme-K, which helps suppress T-cell growth.
- - A diffusion map analysis indicates a gradual differentiation of CD4 T-cells from a naïve state to cytotoxic functions, highlighting EOMES Th1-cells as potential precursors to Tr1-cells.
Article Synopsis
- IFNγ-producing ex-Th17 cells, referred to as Th1/17, are significant in causing experimental colitis and are prevalent in the intestines of patients with Crohn's disease (CD).
- A novel subset of Th17 cells, known as CCR5+ Th17 (pTh17), was identified in human intestines, co-expressing T-bet and RORC/γt, and was found to be linked to intestinal inflammation in CD, particularly responsive to the bacteria Escherichia coli associated with CD.
- Successful treatments, like anti-TNF therapy and anti-IL-23 therapy, reduced pTh17 cell levels, highlighting their role as pro-inflammatory and potentially pathogenic in the context
Mesenchymal stromal cells (MSCs) have been employed in vitro to support hematopoietic stem and progenitor cell (HSPC) expansion and in vivo to promote HSPC engraftment. Based on these studies, we developed an MSC-based co-culture system to optimize the transplantation outcome of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 gene-edited (GE) human HSPCs. We show that bone marrow (BM)-MSCs produce several hematopoietic supportive and anti-inflammatory factors capable of alleviating the proliferation arrest and mitigating the apoptotic and inflammatory programs activated in GE-HSPCs, improving their expansion and clonogenic potential in vitro.
View Article and Find Full Text PDFCancer cells within a tumour have heterogeneous phenotypes and exhibit dynamic plasticity. How to evaluate such heterogeneity and its impact on outcome and drug response is still unclear. Here, we transcriptionally profile 35,276 individual cells from 32 breast cancer cell lines to yield a single cell atlas.
View Article and Find Full Text PDF