Identifying Sequence Effects on Chain Dimensions of Disordered Proteins by Integrating Experiments and Simulations.

It has become increasingly evident that the conformational distributions of intrinsically disordered proteins or regions are strongly dependent on their amino acid compositions and sequence. To facilitate a systematic investigation of these sequence-ensemble relationships, we selected a set of 16 naturally occurring intrinsically disordered regions of identical length but with large differences in amino acid composition, hydrophobicity, and charge patterning. We probed their conformational ensembles with single-molecule Förster resonance energy transfer (FRET), complemented by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy as well as small-angle X-ray scattering (SAXS).

Alzheimer's disease-associated CD83(+) microglia are linked with increased immunoglobulin G4 and human cytomegalovirus in the gut, vagal nerve, and brain.

Introduction: While there may be microbial contributions to Alzheimer's disease (AD), findings have been inconclusive. We recently reported an AD-associated CD83(+) microglia subtype associated with increased immunoglobulin G4 (IgG4) in the transverse colon (TC).

Methods: We used immunohistochemistry (IHC), IgG4 repertoire profiling, and brain organoid experiments to explore this association.

Classical View on Nonclassical Crystal Growth in a Biological Setting.

Crystallization by amorphous particle attachment, a nonclassical crystal growth mode, is prevalent in minerals formed by living tissues. It allows the organism to intervene at every step of crystal growth, i.e.

Plasticity in Hydraulic Architecture: Riparian Trees Respond to Increased Temperatures With Genotype-Specific Adjustments to Leaf Traits.

Climate means and variability are shifting rapidly, leading to mismatches between climate and locally adapted plant traits. Phenotypic plasticity, the ability of a plant to respond to environmental conditions within a lifetime, may provide a buffer for plants to persist under increasing temperature and water stress. We used two reciprocal common gardens across a steep temperature gradient to investigate plasticity in six populations of Fremont cottonwood, an important foundation tree species in arid riparian ecosystems.