Independence of Centromeric and Pericentromeric Chromatin Stability on CCAN Components.

The chromatin of the centromere provides the assembly site for the mitotic kinetochore that couples microtubule attachment and force production to chromosome movement in mitosis. The chromatin of the centromere is specified by nucleosomes containing the histone H3 variant CENP-A. The constitutive centromeric-associated network (CCAN) and kinetochore are assembled on CENP-A chromatin to enable chromosome separation.

Livestock: Public Interface (Exhibition Livestock/Petting Zoo/Agritourism).

The article discusses the importance of biosecurity in exhibitions, shows, and other public livestock venues, such as petting zoos and agritourism sites. It highlights the risks of disease transmission from animals to humans and between animal groups. The document emphasizes the role of veterinarians in developing biosecurity measures and plans, which should be specific to each venue and reviewed regularly.

Profiling of collagen and extracellular matrix deposition from cell culture using in vitro ExtraCellular matrix mass spectrometry imaging (ivECM-MSI).

While numerous approaches have been reported towards understanding single cell regulation, there is limited understanding of single cell production of extracellular matrix phenotypes. Collagens are major proteins of the extracellular microenvironment extensively used in basic cell culture, tissue engineering, and biomedical applications. However, identifying compositional regulation of collagen remains challenging.

Review of Local Homeless Mortality Efforts: A Call for Standardized Data and Reporting.

Context: There are currently no national estimates of how many people die while unhoused in the US. Local jurisdictions have developed their own approaches for estimating homeless mortality.

Objective: We aimed to examine these local approaches, document what is known about homeless mortality, and summarize local methodologies.