A high-throughput protein tagging toolkit that retains endogenous UTRs for studying gene regulation in Kinetoplastids.

Kinetoplastid parasites cause diseases that threaten human and animal health. To survive transitions between vertebrate hosts and insect vectors, these parasites rely on precise regulation of gene expression to adapt to environmental changes. Since gene regulation in Kinetoplastids is primarily post-transcriptional, developing efficient genetic tools for modifying genes at their endogenous loci while preserving regulatory mRNA elements is crucial for studying their complex biology.

Comparing coupled cluster and composite quantum chemical methods for computing activation energies and reaction enthalpies of radical propagation reactions.

Accurate determination of activation energies and reaction enthalpies is essential for understanding the propagation step in free radical polymerization, as it significantly affects polymer chain length and structure. In this study, we compare DLPNO-CCSD(T) to canonical CCSD(T) for 17 radical addition activation energies and 18 reaction enthalpies from Radom and Fischer's test set. Additionally, we compare the computationally efficient composite methods G3(MP2)-RAD and CBS-RAD against CCSD(T)/aug-cc-pVTZ and DLPNO-CCSD(T)/CBS methods.

The Health and Well-Being of Women in Farming: A Systematic Scoping Review.

Objectives: Health and well-being have long been identified as key issues for investigation within agricultural communities. While myriad studies have been conducted to investigate causation, impact, outcomes and interventions among farming populations, the overall emphasis has been disproportionately weighted away from the experiences of women. This systematic scoping review determines the prevalence of topics, identifies the target populations and geographical locations of studies, outlines methodological approaches to the subject area, and summarizes the key findings and conclusions of the available literature.