Plutonium Content in Soil Fractions of Various Sizes and Estimation of the Risks of the Chernobil Nuclear Power Plant Zone.

Based on the data from the literature the authors analyzed the methods used for the estimation of the risks of plutonium-contaminated areas. The analysis was based on the published data on the measured plutonium concentrations in the air and soil. To calculate plutonium concentrations in the near-surface air layer above the contaminated area a modification of the method of the load estimation from the mass was proposed: instead of the average plutonium specific activity in soil the authors suggested the use of the soil coefficient which consists of the sum of specific activities of every respirable fraction (size 0.

[Conjugates of pancreatic ribonuclease and ligand-free human serum albumin].

Conjugates of pancreatic RNase and ligand-free human serum albumin (LFHSA) have been obtained. The number of hydrophobic binding sites both for initial HSA and LFHSA has been determined by the polarised luminescence method. Interaction between RNase and HSA involves additional electrovalent linkage.

[Interaction of Bacillus intermedius 7P ribonuclease with ligand-free serum albumin].

The efficiency of the binding of RNase 7P molecules to albumin on cocondensation with the aim of producing the prolonged action forms of the enzyme can be increased by using ligand-free human serum albumin (LFHSA). The CD method showed that LFHSA underwent changes of the cooperative character under the action of acid and urea. On potentiometric titration the number of titrated groups of LFHSA decreased with time.

[Permeation of pancreatic ATPase through the blood-brain barrier into the cerebral parenchyma].

Penetration of exogenous RNAases, native and albumin polymer-bound, from circulation into the brain parenchyma was studied. Native pancreatic RNAase was found to extend through the hematoencephalic barrier; maximal enzymatic activity was detected within 15-30 min after administration into a rat tail vein. The enzyme activity was not observed within 90 min both in blood serum and brain tissue.

[Nuclease conjugates with ligand-free serum albumin].

In order to obtain nuclease and human serum albumin (HSA) conjugates with a high enzyme content it is proposed to use a ligand-free HSA. The ligands are removed with the help of a strong anion exchanger. A two-stage procedure of conjugate preparation is proposed.