Low-Coordinate Magnesium Sulfide and Selenide Complexes.

The reactions of [{(NacNac)Mg}] (nacnac = HC(iPrCNDip)) with PhP═O at 100 °C afforded the phosphinate complex [(NacNac)Mg(OPPh)(OPPh)] . Reactions of with PhP═E (E = S, Se) proceeded rapidly at room temperature to low-coordinate chalcogenide complexes [{(NacNac)Mg}(μ-S)] and [{(NacNac)Mg}(μ-Se)] , respectively. Similarly, reactions of NHC═S ((MeCNR)C═S with R = Me, Et, or Pr) with afforded NHC adducts of magnesium sulfide complexes, [{(NacNac)Mg(NHC)}(μ-S){Mg(NacNac)}] , that could alternatively be obtained by adding the appropriate NHC to sulfide complex .

Nosocomial transmission of influenza: A retrospective cross-sectional study using next generation sequencing at a hospital in England (2012-2014).

Background: The extent of transmission of influenza in hospital settings is poorly understood. Next generation sequencing may improve this by providing information on the genetic relatedness of viral strains.

Objectives: We aimed to apply next generation sequencing to describe transmission in hospital and compare with methods based on routinely-collected data.

Ultra-rapid, sensitive and specific digital diagnosis of HIV with a dual-channel SAW biosensor in a pilot clinical study.

Despite widened access to HIV testing, around half of those infected worldwide are unaware of their HIV-positive status and linkage to care remains a major challenge. Current rapid HIV tests are typically analogue risking incorrect interpretation, no facile electronic data capture, poor linkage to care and data loss for public health. Smartphone-connected diagnostic devices have potential to dramatically improve access to testing and patient retention with electronic data capture and wireless connectivity.

Minor groove binder modification of widely used TaqMan hydrolysis probe for detection of dengue virus reduces risk of false-negative real-time PCR results for serotype 4.

Dengue is a vector-transmitted viral infection that is a significant cause of morbidity and mortality in humans worldwide, with over 50 million apparent cases and a fatality rate of 2.5 % of 0.5 million severe cases per annum in recent years.

Use of Whole-Genome Sequencing in the Investigation of a Nosocomial Influenza Virus Outbreak.

Traditional epidemiological investigation of nosocomial transmission of influenza involves the identification of patients who have the same influenza virus type and who have overlapped in time and place. This method may misidentify transmission where it has not occurred or miss transmission when it has. We used influenza virus whole-genome sequencing (WGS) to investigate an outbreak of influenza A virus infection in a hematology/oncology ward and identified 2 separate introductions, one of which resulted in 5 additional infections and 79 bed-days lost.