Publications by authors named "R B Cutler"
Thus far, multiple techniques for single cell analysis have been developed, yet we lack a relatively simple tool to assess DNA and RNA from the same cell at whole-transcriptome and whole-genome depths. Here we present an updated method for physical separation of cytoplasmic RNA from the nuclei, which allows for simultaneous studies of DNA and RNA from the same single cell. The method consists of three steps-(1) immobilization of a single cell on solid substrate, (2) hypotonic lysis of immobilized single cell, and (3) separation of cytosol containing aqueous phase and immobilized nucleus.
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- Scientists created a new method called schPTM to study tiny details of proteins in individual cells, especially how they change after certain treatments.
- This method can identify different protein changes (68 types) and can tell the difference between cells that were treated and those that weren't.
- It helps researchers learn more about how cells respond differently to treatments and understand the complex signals, or "codes," in the proteins.
Time-Restricted Feeding Reduces Atherosclerosis in LDLR KO Mice but Not in ApoE Knockout Mice.
Arterioscler Thromb Vasc Biol
September 2024
Article Synopsis
- Dyslipidemia, a risk factor for cardiovascular disease, can be mitigated by time-restricted feeding (TRF), which limits food intake to a 12-hour window, resulting in reduced weight gain and cholesterol levels in preclinical mouse models.
- In studies involving mice with LDLR mutations, TRF significantly decreased hypercholesterolemia and atherosclerosis by promoting lipid metabolism and excretion, demonstrating potential benefits for heart health.
- The findings suggest that TRF could serve as an effective lifestyle intervention for reducing cardiovascular risks, particularly in individuals with LDLR-related conditions, though it may not be effective for those lacking the ApoE protein.
Article Synopsis
- Scientists found that as we get older, our cells collect a lot of tiny changes called mutations.
- They tested how many mutations human cells can handle without getting sick by using a special chemical and looking closely at the DNA.
- It turns out that cells can have around 60,000 mutations and still grow okay, but too many bad mutations can cause problems for the cell.
Article Synopsis
- * The study found that rozanolixizumab can bind to Fc gamma receptors (FcγRs) and mediate a process called antibody bipolar bridging, which influences macrophage surface proteins, but this effect can be inhibited by the presence of human IgG.
- * Importantly, experiments showed that rozanolixizumab's binding to its receptors did not trigger cellular activation, raising questions about its actual engagement with FcγRs in clinical settings where competing IgG is present.