Publications by authors named "R B Bromberg"
Purpose: Many older dogs (Canis lupus familiaris) develop cognitive impairment. Dog owners often describe impairments in multiple sensory functions, yet the relationships between sensory and cognitive function in older dogs is not well understood.
Methods: We performed assessments of dog vision and hearing, both clinically (n = 91, electroretinography and brainstem auditory evoked potential) and via validated questionnaire (n = 238).
The correct description of quantum scattering places the observed scattering contributions on the Ewald's sphere and its Friedel mate. In electron microscopy, due to the large radius of the Ewald's sphere, these contributions are typically merged during data analysis. We present an approach that separates and factorizes these contributions into inversion-symmetric and inversion-antisymmetric components.
View Article and Find Full Text PDFSingle particle reconstruction (SPR) in cryoEM is an image processing task with an elaborate hierarchy that starts with many very noisy multi-frame images. Efficient representation of the intermediary image structures is critical for keeping the calculations manageable. One such intermediary structure is called a particle stack and contains cut-out images of particles in square boxes of predefined size.
View Article and Find Full Text PDFBackground: Herpes simplex virus (HSV) rarely causes organ-invasive infection. Diagnosis and treatment for such infections are often delayed, and mortality is high. We present the first reported case of disseminated HSV-1 infection in an adult causing liver failure, myocarditis, and encephalitis in a patient who recovered after receiving parenteral acyclovir treatment.
View Article and Find Full Text PDFArticle Synopsis
- The His-tag is a common tool used in protein purification that helps researchers study proteins' functions and structures through affinity chromatography.
- The study found that the presence of the His-tag changes how coproheme decarboxylase interacts with the air-water interface during cryoEM grid preparation, affecting particle orientation.
- Researchers suggest that the His-tag may hide certain protein surfaces that interact with the air-water interface, while the linker connecting the tag to the protein could enhance those interactions, indicating that careful tag design can optimize these effects.