Lysine residues are not required for proteasome-mediated proteolysis of cellular prion protein.

Cellular prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored cell-surface protein. The mature cell-surface PrP is internalized and subsequently degraded by lysosomes. Although, proteasomes are proposed to be involved, the precise mechanism of PrP degradation remains uncertain.

Search for Expression Marker Genes That Reflect the Physiological Conditions of Blossom End Enlargement Occurrence in Cucumber.

Blossom end enlargement (BEE) is a postharvest deformation that may be related to the influx of photosynthetic assimilates before harvest. To elucidate the mechanism by which BEE occurs, expression marker genes that indicate the physiological condition of BEE-symptomatic fruit are necessary. First, we discovered that preharvest treatment with a synthetic cytokinin, N-(2-Chloro-4-pyridyl)-N'-phenylurea (CPPU), promoted fruit growth and suppressed BEE occurrence.

Comparison of qualitative and fully automated quantitative tools for classifying severity of white matter hyperintensity.

Objective: In this study, we aimed to compare the Fazekas scoring system and quantitative white matter hyperintensity volume in the classification of white matter hyperintensity severity using a fully automated analysis software to investigate the reliability of quantitative evaluation.

Materials And Methods: Patients with suspected cognitive impairment who underwent medical examinations at our institution between January 2010 and May 2021 were retrospectively examined. White matter hyperintensity volumes were analyzed using fully automated analysis software and Fazekas scoring (scores 0-3).

[Echo Train Length (ETL) of Fluid-attenuated Inversion Recovery (FLAIR) and Extraction Volume of White Matter Hyperintensity Volume in Automated White Matter Signal Analysis].

Purpose: To investigate whether the volume of white matter hyperintensity (WMH) extracted from FLAIR images changes when the imaging parameters of the original images are changed.

Methods: Seven healthy volunteers were imaged by changing the imaging parameter ETL of FLAIR images, and WMHs were extracted and their volumes were calculated by the automatic extraction software. The results were statistically analyzed to examine the relationship (Experiment 1).

Central residues in prion protein PrP are crucial for its conversion into the pathogenic isoform.

Conformational conversion of the cellular prion protein, PrP, into the amyloidogenic isoform, PrP, is a key pathogenic event in prion diseases. However, the conversion mechanism remains to be elucidated. Here, we generated Tg(PrPΔ91-106)-8545/Prnp mice, which overexpress mouse PrP lacking residues 91-106.