Synthesis and characterization of novel chitosan derivatives (containing dipyridinium quaternary salts) with antimicrobial potential.

Chitosan derivatives are versatile materials, biocompatible and biodegradable, that can be tailor-made to suit specific biomedical applications. In this study, two N-heterocyclic salts (N,N'-diphenacyl-[4,4'-dipyridinium] dibromide (DP) and N,N'-diphenacyl-1,2-bis-(4-pyridinium)ethane dibromide (DPE)) were used for chitosan functionalization to enhance its antimicrobial potential. Physico-chemical characterization of the newly synthesized derivatives (Ch-DP and Ch-DPE) was performed by elemental analysis, spectrometry (UV-Vis, FTIR), electrochemistry (OCP, CV), and electron microscopy (SEM) proving that the highest degree of functionalization was obtained for Ch-DP.

Highly sensitive detection of glucose via glucose oxidase immobilization onto conducting polymer-coated composite polyacrylonitrile nanofibers.

Current study introduces composite polyacrylonitrile - multiwall carbon nanotubes nanofibers (PAN-MWCNTs NFs) coated with conducting polymers (polypyrrole (PPy) or poly(3,4-ethylenedioxythiophene) (PEDOT)) by chemical vapor deposition for efficient glucose detection. The potential of nanofibrous assemblies and nano-conducting elements in biosensing was explored as pre-processing of NFs with MWCNTs and post-processing with CPs were both employed. These 'core-shell' conducting NFs were further employed as platforms for glucose oxidase immobilization for enzymatic detection of glucose.

Trace-Level Phenolics Detection Based on Composite PAN-MWCNTs Nanofibers.

In view of major concerns regarding toxicity (genotoxic, mutagenic, hepatotoxic) of phenolics, there is an on-going necessity for sensitive and accurate analytical procedures for detection and measurements in environmental field, water, and food quality control. The current study proposes composite polyacrylonitrile nanofibrous assemblies enriched with multi-wall carbon nanotubes (PAN-MWCNTs NFs) as suitable immobilization platforms for cross-linking of tyrosinase in detection of both diphenols and monophenols, which are of much interest in water contamination. The prepared biosensor (Pt/PAN-MWCNTs/Tyr) showed high sensitivity in catechol detection (362.

Next step in 2nd generation glucose biosensors: Ferrocene-loaded electrospun nanofibers.

Glucose determination is one of the most common analyses in clinical chemistry. Employing biosensors for this purpose has become the method of choice for home use for diabetic patients. To limit the impact of dissolved O concentration or possible interferences (known hindrances in the classical glucose detection approach), a variety of mediated pathways have been explored.

The influence of sugar-protein complexes on the thermostability of C-reactive protein (CRP).

The purpose of the present study was to evaluate de influence of protein-sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar-protein interaction was investigated using steady state and time resolved fluorescence.