Publications by authors named "R A Zubarev"
Article Synopsis
- - Huanglongbing disease (HLB) is a serious threat to the 'Kinnow' mandarin in Pakistan, caused by a bacterial species and spread by the Asian citrus psyllid insect.
- - The study explored the use of selenium nanoparticles (SeNPs) synthesized from garlic extract to enhance fruit quality and antioxidant defenses in HLB-infected 'Kinnow' plants, finding that a 75 mg/L concentration significantly improved various fruit characteristics and antioxidant enzyme levels.
- - Proteomics analysis showed that SeNPs affected the expression of proteins related to growth and stress response, indicating that their application can improve the nutritional quality and overall health of HLB-affected mandarin fruit.
The proteome integral solubility alteration (PISA) assay is widely used for identifying drug targets, but it is labor-intensive and time-consuming and requires a substantial amount of biological sample. Aiming at enabling automation and greatly reducing the sample amount, we developed one-pot time-induced (OPTI)-PISA. Here, we demonstrate OPTI-PISA performance on identifying targets of multiple drugs in cell lysate and scaling down the sample amount to sub-microgram levels, making the PISA method suitable for NanoProteomics.
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