Publications by authors named "R A Yunes"
Article Synopsis
- The study focuses on how gut microbiota, particularly commensal bacteria like lactobacilli, interact with the host immune system's cytokines, which are critical in managing inflammation and gut health.
- Using next-generation RNA sequencing, researchers examined the transcriptional responses of lactobacillus strains K32 and R19-3 to various cytokines, revealing significant changes in gene expression linked to metabolism and stress response, especially upon exposure to IL-8 and IL-10.
- The findings highlight a complex adaptation mechanism where these bacteria adjust their gene expression in response to inflammatory signals, paving the way for potential probiotic therapies for conditions like inflammatory bowel disease (IBD).
Background: Recent advancements in next-generation sequencing (NGS) technology have ushered in significant improvements in sequencing speed and data throughput, thereby enabling the simultaneous analysis of a greater number of samples within a single sequencing run. This technology has proven particularly valuable in the context of microbial community profiling, offering a powerful tool for characterizing the microbial composition at the species level within a given sample. This profiling process typically involves the sequencing of 16S ribosomal RNA (rRNA) gene fragments.
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- This study investigates the ability of specific protein fragments (2D FN3 and CD FN3) from a bacterial strain, GT15, to bind to the inflammatory cytokine TNF-α, using a sandwich ELISA method for detection.
- Results reveal that neither 2D FN3 nor CD FN3 can bind to TNF-α alone, however, the full ∆FN3.1 protein is capable of forming a binding pocket for TNF-α.
- Additionally, the study analyzes genetic variations in the amino acid sequences of ∆FN3.1 and compares FN3 domains from human gut bacteria, highlighting those with cytokine receptor motifs and their structural similarities with human proteins.
Background And Aim: Prolonged stress causes deleterious effects on both the organism and its microbiota. In this study, we examined the effects of exposure to variable frequency ultrasound (US) on the gut microbiota-liver-brain axis of mice.
Materials And Methods: This study was conducted on 20 mature clinically healthy sexually naive C57BL/6J male mice (42-45 days old).
Alzheimer's disease (AD) is a neurodegenerative disease that is characterized as the main dementia in the elderly. Eighteen pyrazolines were synthesized and evaluated for their inhibitory effects against acetylcholinesterase (AChE) . Possible interactions between pyrazolines and the enzyme were explored by experiments.
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