A multiplex serum protein assay for determining the probability of colorectal cancer.

Our purpose is to develop a serum assay to determine an individual's probability of having colorectal cancer (CRC). We have discovered a protein panel yielding encouraging, clinically significant results. We evaluated 431 serum samples from donors screened for CRC by colonoscopy.

Improved image analysis workflow for 2-D gels enables large-scale 2-D gel-based proteomics studies--COPD biomarker discovery study.

2-D gel electrophoresis has been used for more than three decades to study the protein complement of organisms, tissues, and cells. Three issues are holding back large-scale proteomics studies: low-throughput, high technical variation, and study designs lacking statistical power. We identified image analysis as the central factor connecting these three issues.

Overcoming technical variation and biological variation in quantitative proteomics.

Quantitative proteomics investigates physiology at the molecular level by measuring relative differences in protein expression between samples under different experimental conditions. A major obstacle to reliably determining quantitative changes in protein expression is to overcome error imposed by technical variation and biological variation. In drug discovery and development the issue of biological variation often rises in concordance with the developmental stage of research, spanning from in vitro assays to clinical trials.

Probing the molecular physiology of the microbial organism, Escherichia coli using proteomics.

Genomics and proteomics technologies have yielded volumes of data for more than 20 years, and they continues to produce data at an astounding rate. Has all of this data helped us understand more about life, or it is just bogging us down in details that cannot be assembled into meaningful ideas? This review of the proteomics efforts over the last couple of decades is meant to emphasize that a new scientific discipline has emerged, Molecular Physiology, and that, indeed, this discipline is contributing to our understanding of life. Molecular physiology offers the reductionisms details of individual cellular molecules and offers the systems biology multivariant and high-dimensional datasets of cellular molecules.