[Bordetella pertussis agglutinogens in cultivation dynamics].

Bordetella pertussis growth phases during homogenous batch dynamic cultivation in the liquid medium as well as during the static cultivation on the solid medium were established. The maximal activity of agglutination reaction with antisera to B. pertussis agglutinogens 1, 2, and 3 was detected in bacterial culture at the end of exponential phase of growth.

[The effect of methylated cyclodextrin on pertussis toxin accumulation in a Bordetella pertussis culture in a bioreactor].

The results obtained in the study of the influence methylated cyclodextrin (beta CD) on the growth of B. pertussis and the accumulation of pertussis toxin in the course of submerged batch cultivation in a bioreactor are presented. As demonstrated by these results, the presence of beta CD in the culture medium in a dose of 0.

[Immunobiologic activity of Bordetella pertussis strains defective in various virulence factors].

The immunobiological properties of mutant strains, selectively deprived of certain antigens (hemagglutinin, B. pertussis toxin, dermonecrotic toxin, hemolysin, adenylate cyclase), have been studied with the aim of finding out the relationship between the presence of certain antigens in microbial strains and their protective properties. The results of these studies suggest that the protective potency of pertussis vaccine may be related to the presence of some antigenic substances, including those not pertaining to the known factors of virulence.

[Development of a method for the quantitative determination of Bordetella pertussis toxin and the prospects of its use for diagnosis].

Two variants of the enzyme immunoassay (EIA) systems for the determination of B. pertussis toxin (BPT), the "double sandwich" system and the competitive assay system, have been developed. For the titration of BPT in B.