IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Infections.

Introduction: The tools to distinguish relapse from reinfection are needed in malaria-endemic areas. We evaluated seroprevalence against sets of specific peptides to the block 2 region of -merozoite surface protein-1 (PvMSP1) to detect parasite clones.

Methods: We applied amplicon deep sequencing (ADS) of block 2 region of the MSP-1 gene () to determine cocirculating parasite clones within eight -infected individuals.

Evaluating key predictors of anatomical complexity in mandibular incisors: insights from CBCT analysis considering premolar anatomy, sex, and age.

Understanding the impact of premolar root anatomy, sex, and age on incisor complexity can aid in developing tailored diagnostic and therapeutic strategies. This study evaluated if the anatomical complexity of mandibular premolars, sex and age can predict the anatomical complexity in mandibular incisors. Using CBCT scans, 3944 mandibular incisors from 493 patients were analyzed.

Evolutionary dynamics of the B chromosomes in the fish species Valenciennes, 1837 of the Paraná River Basin.

The fish species has an interesting B chromosome system, with three morphological types as acrocentric, metacentric, and submetacentric. However, most cytogenetic studies on this species are restricted to the natural population of the Mogi Guaçu River. Given this, the present work aimed to study the structure karyotypic profile as well as the occurrence of supernumeraries in in several localities in the Paraná River basin, where this species is abundant.

Signature cytokine-associated transcriptome analysis of effector γδ T cells identifies subset-specific regulators of peripheral activation.

γδ T cells producing either interleukin-17A (γδ cells) or interferon-γ (γδ cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ cell versus γδ cell populations from peripheral lymph nodes. Within a very high fraction of differentially expressed genes, we identify a panel of 20 new signature genes in steady-state γδ cells versus γδ cells, which we further validate in models of experimental autoimmune encephalomyelitis and cerebral malaria, respectively.

Distribution of strontium isotopes (Sr/Sr) in surface waters of the Amazon basin: A basis for studies on provenance.

The Sr/Sr isotope ratio has been widely used as an indicator of provenance and migration of aquatic animals, and has applications in other areas such as in environmental and forensic studies. However, the modeling of the spatial distribution in the Amazon basin is still incipient, preventing large-scale applications. In this study, we present a baseline of the Sr/Sr isotope ratio in surface waters of the Amazon basin to infer the provenance and migration patterns of fish.