Publications by authors named "R A Roston"

Article Synopsis
  • The blast fungus Magnaporthe oryzae grows in rice cells through invasive hyphae (IH) and forms a biotrophic interfacial complex, essential for host-pathogen interactions.
  • Disruption of the ACB1 gene, which is vital for fatty acid transport, affects the fungus’s ability to grow and cause disease at lower temperatures (22°C and 26°C) but not at elevated temperatures (29°C).
  • Impaired membrane fluidity due to ACB1 loss at optimal and suboptimal temperatures is responsible for reduced pathogenicity, suggesting that understanding these thermal adaptations is crucial in the context of climate change impacts on plant diseases.
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Low temperatures pose a dramatic challenge to plant viability. Chilling and freezing disrupt cellular processes, forcing metabolic adaptations reflected in alterations to membrane compositions. Understanding the mechanisms of plant cold tolerance is increasingly important due to anticipated increases in the frequency, severity, and duration of cold events.

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In the past few decades, advances in 3D imaging have created new opportunities for reverse genetic screens. Rapidly growing datasets of 3D images of genetic knockouts require high-throughput, automated computational approaches for identifying and characterizing new phenotypes. However, exploratory, discovery-oriented image analysis pipelines used to discover these phenotypes can be difficult to validate because, by their nature, the expected outcome is not known .

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Cotton is an important agricultural crop to many regions across the globe but is sensitive to low-temperature exposure. The activity of the enzyme SENSITIVE TO FREEZING 2 (SFR2) improves cold tolerance of plants and produces trigalactosylsyldiacylglycerol (TGDG), but its role in cold sensitive plants, such as cotton remains unknown. Recently, it was reported that cotton SFR2 produced very little TGDG under normal and cold conditions.

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Chilling stress threatens plant growth and development, particularly affecting membrane fluidity and cellular integrity. Understanding plant membrane responses to chilling stress is important for unraveling the molecular mechanisms of stress tolerance. Whereas core transcriptional responses to chilling stress and stress tolerance are conserved across species, the associated changes in membrane lipids appear to be less conserved, as which lipids are affected by chilling stress varies by species.

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