An Evaluation of Changes in Salivary pH in 3-9-year-old Children Using Chewing Gums (Xylitol) and Lollipops (Xylitol + Erythritol).

Objectives: To evaluate the change in salivary pH using xylitol- and erythritol-containing lollipops and chewing gums, and assess the correlation between sugar substitutes and change in pH levels of saliva.

Materials And Methods: The present crossover study was conducted in 60 children between 3 and 9 years of age from a private school who had not received any meal for 2 hours prior to examination. There were three groups in this study: group I, control group (rinsing with tap water); group II, experimental group (with sugar substitute lollipops); and group III, experimental group (with sugar substitute chewing gums).

Clinical significance of stratifying prostate cancer patients through specific circulating genes.

Patient stratification remains a challenge for optimal treatment of prostate cancer (PCa). This clinical heterogeneity implies intra-tumoural heterogeneity, with different prostate epithelial cell subtypes not all targeted by current treatments. We reported that such cell subtypes are traceable in liquid biopsies through representative transcripts.

Unprecedented Co-occurrence: Identification of a Pathogenic Genetic Variant in the KMT2B Gene in a Wilson Disease Patient with a Pathogenic ATP7B Mutation.

The pathophysiology of dystonia in Wilson disease (WD) is complex and poorly understood. Copper accumulation in the basal ganglia, disrupts dopaminergic pathways, contributing to dystonia's development via neurotransmitter imbalance. Despite advances in diagnosis and management, WD with dystonia remains a challenging condition to treat.

Helical transition of the bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation.

CRISPR-Cas12a is widely used for genome editing and biomarker detection since it can create targeted double-stranded DNA breaks and promote non-specific DNA cleavage after identifying specific DNA. To mitigate the off-target DNA cleavage of Cas12a, we previously developed a Cas12a variant (FnoCas12a ) by introducing double proline substitutions (K969P/D970P) in a conserved helix called the bridge helix (BH). In this work, we used cryogenic electron microscopy (cryoEM) to understand the molecular mechanisms of BH- mediated activation of Cas12a.