[Physico-chemical properties of the thrombolytic compound longolytin].

A preparation exhibiting high fibrinolytic activity and ability to activate plasminogen was isolated from cultivation medium of Arthrobotrys longa. Homogeneous protein, obtained after gel filtration on Sephadex G-100, had molecular mass 28,600, pI-3.68-3.

[Synthesis of sesquiterpene metabolites in a Trichothecium roseum culture].

During the cultivation Trichothecium roseum forms biologically active sesquiterpenes in particular trichothecin and trichothecolon. The quantitative ratio of these compounds in cultural liquid changes depending on the cultivation conditions. The compounds taking part in terpenoids shant specifically increase the synthesis of sesquiterpenes in fungus culture.

[Effect of the enzyme preparation longolytin on fibrinolysis in animals].

It has been established that fibrinolytically active enzyme longolytine isolated from the culture fluid of the saprophyte fungus Arthrobotrys longa at intravenous injection favours the prolonged increase of the plasma fibrinolytic properties as well as activation of endogenic plasminogen. Maximum values of fibrinolytic activity have been marked in 5 and 30 min after enzyme intravenous injection. The plasminogen activity is high in 120 min.

[Anticoagulating properties of fungal proteases tricholysine (triase) and longolytin].

Tricholysine (triase) and longolytin, isolated from cultural fluid of two similar lower fungi Trichothecium roseum and Arthrobotrys longa, exhibited anticoagulant and fibrinolytic properties after addition to blood plasma of experimental animals. Intravenous administration of the enzymes into animals caused the following effects: an increase in fibrinolytic activity, a slight decrease of fibrinogen concentration, an inadequate elongation of recalcification time and of thrombin time. Analysis of thromboelastograms demonstrated the anticoagulant effect: index of coagulation and maximal amplitude were decreased, time of coagulation was elongated.