Colistin resistance and heteroresistance in Klebsiella pneumoniae & Escherichia coli clinical isolates from intensive care units.

Aim: Colistin resistance poses a serious clinical problem. This study aims to investigate the presence of plasmid-mediated resistance and heteroresistance among Escherichia coli (E. coli) and Klebsiella pneumoniae (K.

Prevalence of human papilloma virus (HPV) and its genotypes in cervical specimens of Egyptian women by linear array HPV genotyping test.

Background: The association of human papillomavirus (HPV) with cervical cancer is well established.

Aim: To investigate HPV genotype distribution and co-infection occurrence in cervical specimens from a group of Egyptian women.

Methods: A group of 152 women with and without cervical lesions were studied.

Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms.

Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) gene polymorphisms to verify their ability in assessing the relatedness of isolates. Seventy-five MRSA isolates, from different ICUs of Alexandria University Main Hospital, were characterized using antibiotyping and PCR-RFLP analysis of coa and spa genes.

Quantitative estimation of interferon-gamma levels among Egyptian polytransfused haematology cases.

This study was designed to estimate interferon-gamma (INF-gamma) levels among polytransfused haematology cases. Cases were selected from the haematology unit of Alexandria main university hospital, Egypt. Complete blood counts, estimation of INF-gamma and hepatitis B and C virus (HBV and HCV) status were conducted on 20 unsplenectomized patients with gamma-thalassaemia major and 20 patients with acute myeloid leukaemia (AML) in the maintenance phase and 20 healthy subjects.

Endoscopically directed middle meatal cultures vs computed tomographic scans in the diagnosis of bacterial sinusitis in intensive care units.

Purpose: The objective of the study was to evaluate the accuracy of endoscopically directed middle meatal cultures in the diagnosis of bacterial sinusitis in intensive care unit (ICU) patients in comparison with computed tomographic (CT) scans.

Patients And Methods: The study was conducted at Alexandria University Hospitals on 51 ICU patients with occult fever and clinical signs suggestive of sinusitis. Sinus CT scans were done for all patients, and Calgi swabs were obtained from the middle meatus bilaterally under endoscopic vision using sterile endoscopes with avoidance of any contamination of the swabs.