The drug discrimination assay: Interpretative value of partial generalization for drug schedule control actions.

The U.S. Food & Drug Administration (FDA) has issued a final guidance document on the preclinical determination of abuse potential that must be conducted in animals for all new molecular entities (NMEs) submitted for a new drug application (NDA).

Abuse liability assessment of hydrocodone under current draft regulatory guidelines.

Introduction: The abuse liability of hydrocodone was assessed in male Sprague-Dawley rats under the European Medicines Agency, the International Commission on Harmonisation, and the U.S. Food & Drug Administration draft guidelines for the non-clinical investigation of the dependence potential of medicinal products.

Calretinin expression in the chick cochlear nucleus after deafferentation.

The expression of the calcium-binding protein calretinin (CR) in the chick cochlear nucleus, nucleus magnocellularis (NM), was examined after unilateral cochlea removal in hatchlings and 3-week-old birds. Following survival times of 3, 6 or 12 h or 1, 3 or 7 days, CR mRNA was localized by in situ hybridization using a radiolabeled chick CR probe. Levels of CR protein were assessed by CR-immunoreactivity (CR-I) using a calcium-independent CR antiserum and immunohistochemistry.

Concentration dependence of 19F nuclear magnetic resonance decay shapes and second moments in bone mineral.

19F nuclear magnetic resonance (NMR) spin-echoes and free induction decays (FIDs) have been observed from samples of fluoridated trabecular canine bone powder, with fluoride concentrations ([F]) ranging from approximately 10 to 33 mg F/g Ca. Curve fitting of echo envelopes and FIDs was performed using a two-component model function, where one of the components incorporates the effects of one-dimensional dipolar coupling. This function provides a good match for both echo envelopes and FIDs.

Effects of columella removal on inner ear morphology in the chick.

We are currently removing the single middle ear bone (columella) in the domestic chick to introduce chemical agents directly into the inner ear. Since we are interested in the effect of these agents on neural structures within the avian basilar papilla (BP), we are concerned about any subtle changes that might result from the surgical procedure of columella removal alone. The purpose of this study was to use light and transmission electron microscopy to analyze morphological changes in the inner ear after columella removal.