A review of a colorimetric biosensor based on FeO nanozymes for food safety detection.

The issue of food safety poses a significant threat to human health. The colorimetric sensing method offers a highly sensitive response, visualization, and easy operation, making it highly promising for applications in the field of bioanalysis. FeO nanomaterials not only possess the advantages of a straightforward preparation method, customizable functionalities, and facile surface modification, but also exhibit excellent peroxidase activity.

FSTL1 and TLR4 interact with PEDV structural proteins to promote virus adsorption to host cells.

Infection with porcine epidemic diarrhea virus (PEDV) results in enormous economic damage to the global swine industry. PEDV starts its life cycle by binding to the receptors of host cells and adsorbing onto the cellular surfaces. However, it is still unknown how PEDV adsorbs onto the surface of host cells and the mechanism beneath the interplay of host cell transmembrane protein with PEDV proteins.

Altered neurovascular coupling in semantic variant primary progressive aphasia.

Background: Semantic variant primary progressive aphasia (svPPA) is one of the main clinical phenotypes of frontotemporal lobar degeneration. Changes in both neuronal activity and cerebral perfusion have been observed in svPPA, suggesting a possible breakdown of neurovascular coupling (NVC).

Objective: To investigate alterations in NVC and their correlations with clinical manifestations in svPPA patients.

Single-Atom Catalysts on CN: Minimizing Single Atom Pt Loading for Maximized Photocatalytic Hydrogen Production Efficiency.

The use of metal single atoms (SAs) as co-catalysts on semiconductors has emerged as a promising technology to enhance their photocatalytic hydrogen production performance. In this study, we describe the deposition of very low amounts of Pt SAs (<0.1 at %) on exfoliated graphitic carbon nitride (CN) by a direct Pt-deposition approach from highly dilute chloroplatinic acid precursors.

Expression and Purification of ADAMTSL5 in E. coli and Validation of Activity in Psoriasis Serum.

Purpose: A disintegrin and metalloprotease domain containing thrombospondin type 1 motif-like 5 (ADAMTSL5), a protein linked to psoriasis, was obtained by prokaryotic expression and purification for potential utilization as a new auxiliary diagnostic marker for psoriasis. It was subsequently applied in psoriasis research.

Experimental Design: The designed ADAMTSL5 gene was inserted into the pET-30a (+) vector and expressed in the BL21 (DE3) strain as a fusion protein.

Budding of Asymmetric Lipid Bilayers: Effects of Cholesterol, Anionic Lipid, and Electric Field.

Membrane budding is vital for various cellular processes such as synaptic activity regulation, vesicle transport and release, and endocytosis/exocytosis. Although protein-mediated membrane budding has been extensively investigated, the effects of the lipid asymmetry of the two leaflets and the asymmetrically electrical environments of the cellular membrane on membrane budding remain elusive. In this work, using coarse-grained molecular dynamics simulations, we systematically investigate the impacts of lipid bilayer asymmetry and external electric fields mimicking the asymmetric membrane potential on the membrane budding.

Comparisons of Whole Saliva and Cell Free Saliva by DIA-Based Proteome Profiling.

Background: Saliva has emerged as a promising diagnostic resource due to its accessibility, noninvasiveness, and repeatability, enabling early disease detection and timely intervention. However, current studies often overlook the distinction between whole saliva (WS) and cell-free saliva (CFS). Objective This study aims to compare the proteomic profiles of WS and CFS.

Characterization of seselopsis tianschanica schischk polysaccharide () and its application in developing a functional fermented beverage with highland barle.

This study aimed to isolate and characterize (, a natural functional ingredient, and to develop a compound fermented beverage of nutritional and health combining with highland barley. Firstly, the was isolated and characterized with ultrasound-assisted enzymatic method and chromatography, and analyzed the structural features of polysaccharide -1. Then, a compounded fermented beverage integrating Tibet and highland barley was created, with technology and flavor substances studied.

STING facilitates the development of radiation-induced lung injury via regulating the PERK/eIF2α pathway.

Background: Radiation-induced lung injury (RILI) is one of the serious adverse reactions of thoracic radiotherapy, which largely limits the dose and therapeutic effect of radiotherapy. The underlying mechanism has not been elucidated. RILI is characterized by an acute inflammatory response, and stimulator of interferon genes (STING) has been reported to play an important role in regulating inflammation and innate immune activation.

Oxymatrine Inhibits PD-L1 by Downregulating IFN-γ to Promote Ferroptosis and Enhance Anti-PD-L1 Efficacy in Liver Cancer.

Purpose: Oxymatrine has potent anti-cancer activity, but its exact mechanism in liver cancer remains elusive. The present study was designated to explore oxymatrine's effect and the potential mechanism on Programmed cell death-ligand 1 (PD-L1) expression and ferroptosis in liver cancer.

Methods: Oxymatrine's influence on PD-L1 expression and ferroptosis-related proteins in liver cancer cells was explored in vitro and in vivo utilizing Western blotting, qRT-PCR, immunofluorescence, ELISA, H&E staining, immunohistochemistry, as well as detection of Fe, ROS, and MDA.

Double-target self-supervised clustering with multi-feature fusion for medical question texts.

Background: To make the question text represent more information and construct an end-to-end text clustering model, we propose a double-target self-supervised clustering with multi-feature fusion (MF-DSC) for texts which describe questions related to the medical field. Since medical question-and-answer data are unstructured texts and characterized by short characters and irregular language use, the features extracted by a single model cannot fully characterize the text content.

Methods: Firstly, word weights were obtained based on term frequency, and word vectors were generated according to lexical semantic information.

Language-specific representation of emotion-concept knowledge causally supports emotion inference.

Humans no doubt use language to communicate about their emotional experiences, but does language in turn help humans understand emotions, or is language just a vehicle of communication? This study used a form of artificial intelligence (AI) known as large language models (LLMs) to assess whether language-based representations of emotion causally contribute to the AI's ability to generate inferences about the emotional meaning of novel situations. Fourteen attributes of human emotion concept representation were found to be represented by the LLM's distinct artificial neuron populations. By manipulating these attribute-related neurons, we in turn demonstrated the role of emotion concept knowledge in generative emotion inference.

Bacterial suppressor-of-copper-sensitivity proteins exhibit diverse thiol-disulfide oxidoreductase cellular functions.

Disulfide bond (Dsb) oxidoreductases involved in oxidative protein folding govern bacterial survival and virulence. Over the past decade, oligomerization has emerged as a potential factor that dictates oxidoreductase activities. To investigate the role of oligomerization, we studied three Dsb-like ScsC oxidoreductases involved in copper resistance: the monomeric StScsC, and the trimeric PmScsC and CcScsC.

Exercise intolerance in patients with chronic coronary syndrome: insights from exercise stress echocardiography.

Aims: This study applied exercise stress echocardiography (ESE) to identify risk factors associated with exercise intolerance in patients with chronic coronary syndrome (CCS).

Methods And Results: 90 CCS patients underwent a cardiopulmonary exercise test and ESE, assessing exercise capacity, left ventricular systolic and diastolic function, and systolic reserve. The patients were divided into two groups according to the percentage of predicted oxygen consumption (VO) at peak (≥85%, normal exercise tolerance group; <85%, exercise intolerant group).

The Role of NDRG1 Expression in Vasculogenic Mimicry of High-grade Gliomas.

Glioma is one of the most common primary malignant tumors of the central nervous system, and the prognosis is getting worse with the improvement of tumor grade. In recent years, with the in-depth study of the disease, researchers have found that microvascular mimicry seems to be the key factor of poor prognosis, but its specific mechanism has not been thoroughly studied, and the expression of microvascular mimicry in high-grade gliomas is closely related to the expression of NDRG1 gene. This research project is expected to control the occurrence and development of HGG by exploring the factors that affect the expression of NDRG1 gene.

Exploring the properties of new super-chalcogens based on multiple electron counting rules: a combined DFT and study on [M(BCX)] dianion clusters.

The theoretical exploration of the super-chalcogen properties of multi-charged sandwich structures whose geometry simultaneously satisfyies the octet rule and Hückel's 4+2 rule is reported here a case study of dianion clusters [M(BCX)] (M = Be, Mg or Ca; X = H, F or Cl). The properties of these dianion clusters [M(BCX)] are close to or even superior to those of traditional clusters based on separate electron-counting rules, , the octet rule and Hückel's 4+2 rule. At the theoretical level of combined and DFT methods, these clusters, including halogen-substituents (F, Cl) are super-chalcogens due to their high first vertical electron detachment energy (FVDE), of which the largest value is 1.

Construction and Magnetic Difference of {CoCoM} (M = Co/Cd) Aggregates with Homo- and Heterometallic Centers Derived from {Co} Precursors.

Controllable synthesis of sophisticated aggregations has attracted wide attention from scientists. In this work, homo- and heterometallic {CoCoM} ( = Co/Cd) aggregates with similar skeletons, composed of two [CoM(dpbt)] peripheries and a central [Co(OH)] vertex-fused double-cubane, were constructed from prefabricated {Co} clusters and characterized by single-crystal X-ray diffraction and XPS, ICP-MS, SEM (EDX), and so on. And then, the structure and magnetic differences of them had been further analyzed under dotted with paramagnetic Co ions and/or diamagnetic Cd ions.

Association Between Geriatric Nutritional Risk Index and Critically Ill Patients With Pressure Injury: Analysis of the MIMIC-IV Database.

Aims And Objectives: To explore the relationship between the Geriatric Nutritional Risk Index (GNRI) and the occurrence of Pressure injury (PI) in elderly Intensive Care Unit (ICU) patients.

Background: PI represent a significant health concern within ICU, where the occurrence of such injuries is notably high among critically ill patients. However, few studies have explored the relationship between GNRI and PI.

A 13-years pharmacovigilance analysis of novel hormonal agents in prostate cancer using the FDA adverse event reporting system database.

Background: Novel hormonal agents (NHAs), such as abiraterone, apalutamide, enzalutamide, and darolutamide, play a pivotal role in prostate cancer treatment. However, the toxicity spectrum of NHAs in the real-world has not been fully investigated. This study aims to analyze the adverse events (AEs) linked to NHAs.

The Effects of Ligands with Different Electron Donation on the Nitrogen Reduction Performance of Constructed Co-MOFs: NH or NH?

Four cobalt-based metal-organic frameworks (Co-MOFs) based on different ligands with varying electron-donating properties (dpt = 2,5-di(pyridin-4-yl)-1,3,4-thiadiazole, NH₂bdc = 5-aminoisophthalic acid, OHbdc = 5-hydroxyisophthalic acid, H₂tdc = thiophene-2,5-dicarboxylic acid, and hfipbb = 4,4'-(hexafluoropropane-2,2-diyl)bisbenzoic acid) are synthesized. Among them, Co-dpt-NH₂bdc demonstrates the highest nitrogen reduction reaction activity, achieving an ammonia (NH) yield of 68.61 µg·h⁻¹·mg⁻¹ and Faraday efficiency (FE) of 6.

Knowledge, attitude, and practice of clinical nurses towards medical device-related pressure injury prevention: A systematic review.

Objective: To evaluate clinical nurses' knowledge, attitude, and practice toward MDRPI prevention.

Background: Medical device-related pressure injury (MDRPI) is a significant concern in healthcare, serving as a critical indicator of patient safety and the quality of nursing care.

Methods: This systematic review was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.

Variable-coupled distributed resource allocations for multi-agent system with limited admissible interaction.

Variable-coupled distributed resource allocations (VCDRAs) involve optimizing resource distribution among interconnected entities, reflecting the complex correlation in practical systems. Based on the consensus technology, this paper proposes a novel distributed optimization algorithm to address the VCDRA in scenarios where agents are confronted with admissible interaction ranges and external disturbances. Admissible interaction refers to agents only transmitting information within a specified range due to limited communication capabilities or resource availability.