Interleukin-6 (-174G/C), Interleukin-1β (-511 C/T), and Apolipoprotein B-100 (2488 C/T) Gene Polymorphism in Pre-Eclampsia.

: Pre-eclampsia (PE) is a pregnancy-specific condition characterized by significant health risks for pregnant women worldwide due to its status as a multi-organ disorder. High blood pressure (hypertension) with or without proteinuria is usually considered an initial clinical sign of PE. The pathogenesis of pre-eclampsia is highly complex and likely involves multiple factors, including poorly developed uterine spiral arterioles, immunological issues, placental ischemia or infarction, and genetic abnormalities.

Differential expression of "Candidatus Liberibacter solanacearum" genes and prophage loci in different life stages of potato psyllid.

Psyllid species, including the potato psyllid (PoP) Bactericera cockerelli (Sulc) (Triozidae) serve as host and vector of "Candidatus Liberibacter spp." ("Ca. Liberibacter"), which also infects diverse plant hosts, including citrus and tomato.

Apolipoprotein E Gene Variation in Pakistani Subjects with Type 2 Diabetes with and without Cardiovascular Complications.

Apolipoprotein E (APOE) gene polymorphism has been implicated in the pathogenesis of various metabolic disorders, including type 2 diabetes mellitus (T2DM). Type 2 diabetes mellitus (T2DM) is a major public health concern worldwide, including in Pakistan. Cardiovascular problems linked with T2DM have a significant impact on individuals and society.

ChainAgile: A framework for the improvement of Scrum Agile distributed software development based on blockchain.

Software Development based on Scrum Agile in a distributed development environment plays a pivotal role in the contemporary software industry by facilitating software development across geographic boundaries. However, in the past different frameworks utilized to address the challenges like communication and collaboration in scrum agile distributed software development (SADSD) were notably inadequate in transparency, security, traceability, geographically dispersed location work agreements, geographically dispersed teamwork effectiveness, and trust. These deficiencies frequently resulted in delays in software development and deployment, customer dissatisfaction, canceled agreements, project failures, and disputes over payments between customers and development teams.

Incidence of eriophyid mites (Acariformes: Eriophyidae) and predatory mites (Parasitiformes: Phytoseiidae) in Florida citrus orchards under three different pest management programs.

The abundance and diversity of eriophyid and phytoseiid mites in south and central Florida were assessed in six citrus orchards under three different pest management systems, conventional, organic, and untreated. Tree canopy, ground cover, and leaf litter were sampled every two months in two groves for each of the three pest management systems from April 2019 to February 2021. The citrus rust mite, Phyllocoptruta oleivora (Ashmead) represented 95 to 99% of the rust mites sampled in each grove except in one untreated orchard where it accounted for 45% of the samples (n = 938 total P.

Optimal dsRNA Concentration for RNA Interference in Asian Citrus Psyllid.

The Asian citrus psyllid (ACP) is a citrus pest and insect vector of " Liberibacter asiaticus", the causal agent of citrus greening disease. Double-stranded RNA (dsRNA) biopesticides that trigger RNA interference (RNAi) offer an alternative to traditional insecticides. Standardized laboratory screening of dsRNA requires establishing the minimal effective concentration(s) that result in effective RNAi "penetrance" and trigger RNAi, resulting in one or more measurable phenotypes, herein, significant gene knockdown and the potential for mortality.

Evidence-based position paper on physical and rehabilitation medicine professional practice for persons with COVID-19, including post COVID-19 condition: the European PRM position (UEMS PRM Section).

Although multiple factors still pose challenges to inpatient/outpatient rehabilitation for survivors of COVID-19, rehabilitation plays a key role for this patient population. This study aimed to improve Physical and Rehabilitation Medicine (PRM) physician's professional practice for persons with COVID-19-related functioning limitations, to promote functional recovery and reduce activity limitations and/or participation restrictions. A systematic review of the scientific literature was performed from December 2019 to August 2022, followed by production of recommendations through 5 Delphi rounds, by consensus among the delegates of all European countries represented in the Union of European Medical Specialists PRM Section.

UV-LASER adjuvant-surfactant-facilitated delivery of mobile dsRNA to tomato plant vasculature and evidence of biological activity by gene knockdown in the potato psyllid.

Background: Double-stranded RNA (dsRNA) biopesticides are of interest for the abatement of insect vectors of pathogenic bacteria such as 'Candidatus Liberibacter', which infects both its psyllid and plant hosts. Silencing of genes essential for psyllids, or for Liberibacter, is anticipated to lead to mortality or impeded bacterial multiplication. Foliar delivery is preferred for biopesticide application; however, the cuticle impedes dsRNA penetration into the vasculature.

Species assemblage, abundance, and distribution of Phytoseiid mites (Parasitiformes: Phytoseiidae) in Citrus Under Protective Screen.

Citrus Under Protective Screen (CUPS) production system excludes the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, vector of Huanglongbing (HLB), and large predators. However, small pests and beneficial arthropods such as predatory mites may enter the CUPS. Predatory mites from the family Phytoseiidae attack several economically important pests, especially phytophagous mites common in CUPS.

Feeding behavior and hormoligosis associated with imidacloprid resistance in Asian citrus psyllid, Diaphorina citri.

Imidacloprid is a neonicotinoid insecticide used for managing the Asian citrus psyllid, Diaphorina citri Kuwayama, which serves as vector of phytopathogens causing citrus greening. However, development of resistance to neonicotinoids among populations of D. citri has coincided with occasional control failures in the field.

Evaluation of aquifer parameters through integrated approach of geophysical investigations, pumping test analysis and Dar-Zarrouk parameters in the central part of Bari Doab, Punjab, Pakistan.

In the central part of Bari Doab in Punjab Province of Pakistan, the factors such as sporadic rainfall pattern, decrement of water in rivers, subsurface salinity and excessive mining of groundwater have badly affected the hydrogeology and recharge system of aquifer. The present research work is an endeavour to evaluate the characteristics and potential of aquifer for its future sustainable availability within the study area of central part of Bari Doab. The geophysical studies, pumping tests data, borehole logs and Dar-Zarrouk parameters were used integrally to evaluate the aquifer hydraulic and hydrologic parameters in the study area.

Post COVID-19 complications and follow up biomarkers.

Millions of people were infected by the coronavirus disease (COVID-19) epidemic, which left a huge burden on the care of post COVID-19 survivors around the globe. The self-reported COVID-19 symptoms were experienced by an estimated 1.3 million people in the United Kingdom (2% of the population), and these symptoms persisted for about 4 weeks from the beginning of the infection.

Wild lime psyllid Leuronota fagarae Burckhardt (Hemiptera: Psylloidea) picorna-like virus full genome annotation and classification.

Picorna-like viruses of the order Picornavirales are a poorly defined group of positive-sense, single-stranded RNA viruses that include numerous pathogens known to infect plants, animals, and insects. A new picorna-like viral species was isolated from the wild lime psyllid (WLP), Leuronota fagarae, in the state of Florida, USA, and labelled: Leuronota fagarae picorna-like virus isolate FL (LfPLV-FL). The virus was found to have homology to a picorna-like virus identified in the Asian Citrus Psyllid (ACP), Diaphorina citri, collected in the state of Florida.

Proceeding the categorization of microplastics through deep learning-based image segmentation.

Microplastics (MPs) have been recognized as prominent anthropogenic pollutants that inflict significant harm to marine ecosystems. Various approaches have been proposed to mitigate the risks posed by MPs. Gaining an understanding of the morphology of plastic particles can provide valuable insights into the source and their interaction with marine organisms, which can assist the development of response measures.

Climatic and topographic controls on glacial changes (1973-2020) in Shigar Basin, Central Karakoram, Northern Pakistan.

The Hindukush, Karakorum, and Himalaya (HKH) mountains are often referred to as the "Third Pole" because of high snow, being a major freshwater resource and early indicator of climate change. Therefore, research on the dynamics of glacier changes and their relationship with climate and topographic variability is essential for sustainable water resource management and adaptation strategies in Pakistan. In this contribution, we delineated 187 glaciers and examined these glacier changes in the Shigar Basin from 1973 to 2020 using Corona, Landsat Operational Land Imager/Enhanced Thematic Mapper Plus/Thematic Mapper/Multispectral Scanner System (OLI/ETM/TM/MSS), Alaska Satellite Facility (ASF), and Shuttle Radar Topography Mission Digital Elevation Model (SRTM DEM) imageries.

Tumour infiltrating lymphocytes in colorectal cancers- correlation with tumour biology and clinical outcome: A cohort study.

Objective: To evaluate the pattern of tumour infiltrating lymphocytes in colorectal cancers, and to correlate them with nuclear protein Ki67, vascular endothelial growth factor and clinical outcome.

Methods: The retrospective study was conducted at the Nuclear Institute of Medicine and Radiotherapy and the Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan, and comprised data of colorectal cancer patients from January 1, 2008, to December 31, 2018. Whole tumour sections of colorectal cancer were used with haematoxylin and eosin staining, Histological type, grade and infiltrated lymphocytes within the tumour block were assessed.

Formulation, optimization and in vitro evaluation of sustained release oral hydrogels of diacerein to treat arthritis.

The current study is aimed to formulate pH responsive polymeric hydrogels. Potassium per sulphate and Methylene bis acrylamide were employed as initiator and cross linker respectively. To determine the effect of substrate on degree of cross linking different ratios of the acrylic acid (AA), potassium per sulphate (KPS) and methylenebisacrylamide (MBA) were used.

Annotation of putative circadian rhythm-associated genes in (Hemiptera: Liviidae).

The circadian rhythm involves multiple genes that generate an internal molecular clock, allowing organisms to anticipate environmental conditions produced by the Earth's rotation on its axis. Here, we present the results of the manual curation of 27 genes that are associated with circadian rhythm in the genome of the Asian citrus psyllid. This insect is the vector for the bacterial pathogen Liberibacter asiaticus (Las), the causal agent of citrus greening disease (Huanglongbing).

Consortium Framework Using Blockchain for Asthma Healthcare in Pandemics.

Asthma is a deadly disease that affects the lungs and air supply of the human body. Coronavirus and its variants also affect the airways of the lungs. Asthma patients approach hospitals mostly in a critical condition and require emergency treatment, which creates a burden on health institutions during pandemics.

Sesame as an Alternative Host Plant to Establish and Retain Predatory Mirids in Open-Field Tomatoes.

The silverleaf whitefly (Bemisia tabaci) and the South America tomato pinworm (Tuta absoluta) are two of the most destructive pests of tomato. Open-field tomato production frequently relies on chemical treatments, which has been shown to lead to pesticide resistance. The integration of biological control using predatory mirid bugs is an effective alternative method for managing these pests.

Association of CARD14 Single-Nucleotide Polymorphisms with Psoriasis.

Psoriasis is an immune-mediated chronic and painful disease characterized by red raised patches of inflamed skin that may have desquamation, silvery-white scales, itching and cracks. The susceptibility of developing psoriasis depends on multiple factors, with a complex interplay between genetic and environmental factors. Studies have suggested an association between autosomal dominant CARD14 (caspase recruitment domain-containing protein 14) gain-of-function mutations with the pathophysiology of psoriasis.

Human Coronavirus Spike Protein Based Multi-Epitope Vaccine against COVID-19 and Potential Future Zoonotic Coronaviruses by Using Immunoinformatic Approaches.

Zoonotic coronaviruses (CoV) have emerged twice and have caused severe respiratory diseases in humans. Due to the frequent outbreaks of different human coronaviruses (HCoVs), the development of a pan-HCoV vaccine is of great importance. Various conserved epitopes shared by HCoVs are reported to induce cross-reactive T-cell responses.

Integrated Water, Nutrient, and Pesticide Management of Huanglongbing-Affected Sweet Oranges on Florida Sandy Soils-A Review.

Citrus greening (huanglonbing (HLB)) disease has drastically reduced citrus fruit production in Florida over the last two decades. Scientists have developed sustainable nutrient management practices to live with the disease and continue fruit production. Best pesticide management practices have been devised to reduce the spread of HLB by Asian citrus psyllid (ACP).

Novel molecular classification of colorectal cancer and correlation with survival.

Introduction: Colorectal cancer (CRC) is one of the most common cancers worldwide. This study was designed to evaluate biological patterns, explore molecular classification and correlate with survival outcome in treatment naïve CRC patients.

Methods: Over 11 years consecutive series of 435 CRC patients were operated on as primary surgical therapy.