Publications by authors named "Qun-li Wang"
Crocetin induces cytotoxicity in colon cancer cells via p53-independent mechanisms.
Asian Pac J Cancer Prev
April 2013
Article Synopsis
- Crocin is being studied for its potential as a cancer chemopreventive agent, particularly against human colon cancer cells.
- The study utilized various techniques, such as MTT assays, flow cytometry, and Western blot analysis, to assess cell proliferation, apoptosis, and DNA damage.
- Results indicated that crocetin significantly inhibited cancer cell growth, induced cell cycle arrest, and enhanced apoptosis, suggesting its potential use in cancer treatment.
Imperatorin (Imp) as a hypotensive active ingredient, its hypotensive effect was evaluated in the SHRs, its calcium antagonism and affinity to L-type calcium channel was also confirmed. The results showed that the blood pressure was decreased in the SHRs treated with Imp, the aortic ring was relaxed with Imp, L-type calcium channel currents and intracellular calcium free ion rise was nearly disappeared when adding Imp. In addition, Imp displayed a chromatographic peak similar to nitrendipine and verapamil by the cell membrane chromatography, same results from protein-drug docking approaches.
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- Store-operated Ca(2+) entry (SOCE) is thought to play a role in calcium influx in vascular smooth muscle cells, and the study explored how this relates to the vasodilatory effects of imperatorin, a dietary compound.
- The researchers conducted experiments on rat mesenteric arteries and VSMCs, using both measurement of isometric tension and confocal microscopy to assess calcium levels.
- Despite imperatorin causing vasodilation, the study found that it does not inhibit SOCE-induced calcium increases or contractions, suggesting that the vasodilation effect is not due to the SOCE mechanism, and it shares similarities with the effects of nifedipine.
[Green fluorescent protein as a tracer of bone marrow stromal cells in bone tissue engineering in rhesus].
Nan Fang Yi Ke Da Xue Xue Bao
February 2007
Objective: To observe the role of green fluorescent protein (GFP) in tracing rhesus bone marrow stromal cells (rBMSCs) during tissue-engineered bone formation in vivo.
Methods: Ad5.CMV-GFP was amplified by infecting QBI-293A cells, and the bone marrow was harvested from the ilium of adult male rhesus to obtain rBMSCs, which were cultured and passaged in vitro.
[Optical, scanning electron and atomic force microscopic observation of the microstructure of human humeral bone].
Di Yi Jun Yi Da Xue Xue Bao
April 2005
Objective: To observe the microstructure of human long bone with scanning electron and atomic force microscopes to understand the ultrastructural organization and the composition and the morphology of the bone collagen and minerals.
Method: Fresh human cadaveric humeral bone were fixed and dehydrated to prepare the ground sections and thin slices, respectively, which were observed for bone microstructure using optical, scanning electron and atomic force microscopy, respectively.
Results: Under optical microscope, the bone lacunae were in circular permutation around the Haversian canal, and the canaliculi communicated the lacunae and Haversian canal or between the lacunae.
Objective: To study the in vitro biocompatibility of novel hydroxyapatite (HA) and AO artificial bone beta-tricalcium phosphate (beta-TCP) with rhesus bone marrow stromal cells (rBMSCs) .
Methods: The third passage of rBMSCs were cultured with HA and beta-TCP respectively, with the cells cultured without the materials as the control. The morphology and proliferation of cells were observed by inverted phase-contrast microscope and scanning electron microscope (SEM).
Objective: To study the effect of ischemic preconditioning (PC) and pharmacological preconditioning with adenosine or diazoxide on ischemia-reperfusion (IR) injury in the limbs of rats.
Methods: According to different treatment received before ischemic-reperfusion injury, 66 SD rats were divided into 6 groups including a normal control and a ischemia-reperfusion control group, IP10 group in which the rats received 10-min ischemia followed by 10-min interval for reperfusion for 3 times before IR, IP5 group in which the rats were subjected to 5-min ischemia with 5-min reperfusion intervals for 3 times before IR, adenosine (Ade) pretreatment group and diazoxide (Dia) pretreatment group. Except the normal control group, which consisted of 6 rats, each group contained 12 rats, and IR injury was induced by blocking the blood flow in bilateral limbs for 4 h, followed by reperfusion for 2 or 24 h when twitch and spastic contractility of the tibialis anterior muscle and serum creatine phosphokinase (CPK) were measured.