Publications by authors named "Quiles-Melero I"
Article Synopsis
- Respiratory infections significantly affect hospitalization rates and costs for children with tracheostomies, prompting a study on antibiotic treatment patterns.
- The research analyzed medical records of 83 children to evaluate antibiotic use for tracheobronchitis and nonspecific respiratory episodes (NSRE), revealing that a high percentage of tracheobronchitis cases received antibiotics, while many NSRE cases did not require them.
- Factors like fever were linked to increased antibiotic prescriptions, but many cases resolved without treatment, indicating the need for careful antibiotic prescription practices to avoid unnecessary usage.
Clinical Utility of a Multiplex PCR Panel (BioFire Joint Infection) in the Adjustment of Empiric Antimicrobial Therapy: Experience in Pediatric Osteoarticular Infections.
Children (Basel)
October 2024
Background/objectives: This study aims to evaluate the impact of the PCR multiplex panel (BioFire JI) on the diagnosis and management of pediatric osteoarticular infections.
Methods: This retrospective study analyzed data from pediatric patients diagnosed with osteoarticular infections between January 2023 and April 2024. The effectiveness of the PCR multiplex panel in identifying pathogens was compared with traditional culture methods.
Utility of culture and molecular methods using Allplex Bacterial Vaginosis Plus Assay (Seegene) as a tool for endometriosis, infertility and recurrent pregnancy loss diagnosis.
Diagn Microbiol Infect Dis
November 2024
Endometriosis, infertility, or recurrent pregnancy loss (RPL) are entities characterised by a decrease in Lactobacillus spp. and an increase in bacterial vaginosis-associated bacteria, (BVAV) according with 16S rRNA sequencing studies. However, the use of nucleic acid amplification tests (NAAT) as a tool for diagnosis algorithms is unknown.
View Article and Find Full Text PDFGlobally, an increase in sexually transmitted diseases worldwide has been reported, including acquired infection. Congenital syphilis is the mother-to-child transmission syphilis infection, experiencing an increase in cases returning to incidence rates of years ago. We report three cases of symptomatic congenital syphilis occurring in a tertiary-care hospital in Madrid (Spain).
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- This study aims to quickly and accurately detect the Y132F ERG11p mutation in Candida parapsilosis isolates and to analyze genetic diversity among these isolates for better tracking of fluconazole resistance.!* -
- The research involved 223 isolates from patients across 20 hospitals in Spain and Italy, using newly optimized PCR methods that require no DNA extraction and provide results in as little as 1.5 hours.!* -
- The findings confirm both PCR methods have 100% specificity and sensitivity, with Scheme 1 showing greater genetic diversity compared to Scheme 2, suggesting the use of specific microsatellite markers for better genotyping of resistant strains.!*
Background: Despite respiratory infections being a leading cause of hospitalization in children with tracheostomy tubes, there are no published guidelines for their diagnosis and management. This study aims to outline the clinical, laboratory and microbiological aspects of pneumonia in these children, along with the antibiotics used and outcomes. Additionally, it seeks to determine pneumonia incidence and associated risk factors.
View Article and Find Full Text PDFArticle Synopsis
- - An increase in gonococcal infections has been observed in Europe and Spain, leading to more cases of disseminated gonococcal infection, which can include gonococcal arthritis.
- - Five cases of gonococcal arthritis were reported in a Madrid hospital between October 2022 and October 2023, primarily affecting male patients with a history of unprotected sex.
- - Advances in molecular techniques have improved the identification of culture-negative gonococcal arthritis cases and have revealed mutations linked to fluoroquinolone resistance, prompting a shift towards oral treatments.
Over the past few years, venereal or sexually transmitted infections (STIs) have been on the rise worldwide requiring additional specialized monographic consultations to specifically treat STIs. Therefore, the Spanish Academy of Dermatology and Venereology (AEDV) Research Working Group on STIs and HIV has drafted this document with the necessary requirements in terms of infrastructure, personnel, technology, specific materials for sample collection, and needs for current therapeutic options. Strict emphasis is placed on the protection of patient privacy.
View Article and Find Full Text PDFBackground: Fluconazole-resistant Candida parapsilosis is a matter of concern.
Objectives: To describe fluconazole-resistant C. parapsilosis genotypes circulating across hospitals in Spain and Rome and to study their azole-resistance profile associated with ERG11p substitutions.
Comparison of gradient diffusion and molecular methods using Allplex™ NG&DR assay (Seegene®) for macrolide and fluoroquinolone screening resistance in Neisseria gonorrhoeae.
Eur J Clin Microbiol Infect Dis
May 2024
Article Synopsis
- * The study compared a gradient diffusion test to a new molecular method (Allplex™ NG&DR assay) for detecting mutations linked to resistance in 100 NG isolates, finding no significant correlation for fluoroquinolone resistance but identified a high rate of S91F mutations.
- * While the molecular method showed good sensitivity (96.1%) and specificity (90.9%) for fluoroquinolone resistance, it cannot replace the gradient diffusion test for macrolides due to lack of positive results for resistance mutations.
Article Synopsis
- - The study focused on understanding the clinical impact of filamentous basidiomycetes found in respiratory samples, which are often seen as environmental contaminants.
- - Researchers analyzed respiratory sample data from a tertiary care hospital between 2020 and 2023, identifying six unique basidiomycete species in immunosuppressed patients, all of whom improved without antifungal treatment.
- - The findings suggest that while the clinical significance of these fungi remains unclear, their presence in at-risk patients warrants further investigation, particularly concerning possible allergic reactions or invasive infections.
[Translated article] Descriptive study of spinal instrumentation-related infections in a tertiary hospital.
Rev Esp Cir Ortop Traumatol
January 2024
Introduction: Spinal instrumentation-related infections (SIRI) are one of the main causes of post-surgical complication and comorbidity. Our objective was to describe the clinical and microbiological characteristics, treatment and prognosis of these infections.
Material And Methods: We conducted a retrospective study in our institution (2011-2018) including adult patients undergoing spinal instrumentation who met the diagnostic criteria for confirmed infection.
Traditionally, lymphogranuloma venereum (LGV) has been associated with disease of the genital area. However, atypical presentations and proctitis are increasingly observed. We report a case of LGV affecting the dorsum of the tongue, which presented as a very painful ulcer.
View Article and Find Full Text PDFArticle Synopsis
- The study builds on findings from the CANDIMAD study (2019-2021) regarding antifungal resistance in Madrid, revealing trends of increased fluconazole resistance in 2022 among isolates from blood cultures and intra-abdominal samples.
- A total of 766 isolates were collected, with a notable rise in fluconazole resistance (12.0% overall, 16.0% in blood cultures), largely attributed to specific resistant clones, while resistance to other antifungals remained low.
- The data indicates a concerning spread of fluconazole-resistant genotypes in Madrid, with up to 7 out of 16 hospitals reporting affected cases, highlighting the need for vigilance in antifungal treatment strategies.
Objective: The prevalence of drug-resistant Neisseria gonorrhoeae (NG) infections is increasing. Studies report the prevalence of NG strains presenting A2059G/C2611T (rRNA23S) and S91F (parC) mutations conferring resistance to azithromycin and ciprofloxacin.
Methods: We conducted a prospective cohort study evaluating first void-urine urines, rectal, and oropharyngeal swabs collected from a cohort of patients in a tertiary hospital in Madrid between October 2022 and January 2023.
Descriptive study of spinal instrumentation-related infections in a tertiary hospital.
Rev Esp Cir Ortop Traumatol
September 2023
Introduction: Spinal instrumentation-related infections (SIRI) are one of the main causes of post-surgical complication and comorbidity. Our objective was to describe the clinical and microbiological characteristics, treatment and prognosis of these infections.
Material And Methods: We conducted a retrospective study in our institution (2011-2018) including adult patients undergoing spinal instrumentation who met the diagnostic criteria for confirmed infection.
Objectives: Antifungal susceptibility testing is mostly conducted on blood-cultured Candida spp isolates. Because the intra-abdominal cavity has been highlighted as a hidden echinocandin-resistant C. glabrata reservoir, we assessed whether testing sequential isolates from a given patient might increase the chances of detecting antifungal resistance.
View Article and Find Full Text PDFObjective: Mycoplasma genitalium causes persistent sexually transmitted infections. The aims of this study were to estimate the prevalence of resistances to macrolides and fluoroquinolones in M. genitalium and the sexually transmitted coinfections in patients at Hospital Universitario La Paz (Madrid, Spain).
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