Publications by authors named "Quesada A"

To determine the effects of exercise training on exercise-induced ischemia in patients following myocardial infarction, the experience of 13 patients with exercise-induced ST depression, who were moderate-to-high intensity trained for 1 year, has evaluated. After training, the maximum ST depression was significantly less (1.9 +/- 0.

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The following two species are described from Carduelis sinica (Greenfinch) from Italy. The oocysts of Isospora mcquistioni n. sp.

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Native vesicles isolated from Ehrlich ascites tumor cells accumulate glutamine by means of Na(+)-dependent transport systems; thiocyanate seems to be the more effective anion. The apparent affinity constant for the process was 0.38 mM.

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We studied the hemodynamic behavior of the pulmonary circulation at rest and during exercise in six patients with MTB. As a group, in contrast to advanced fibrocaseous tuberculosis, these patients exhibited normal pulmonary hemodynamics at rest and during exercise. Only minor abnormalities in pulmonary vascular resistance at exercise (increased PAd-PWP gradient) were noted in two of the patients.

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In this present study we analyse, with the help of the Golgi method, the effect of ethanol on the morphological differentiation of the cerebellar granule cells in the chick embryo. Ethanol seems to affect the process of cell migration from the early stages of differentiation. Some granule cells appear to differentiate in an inverted position.

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The effect of ethanol on the cerebellar cortex of chick embryos was studied in semi-thin sections of material prepared for electron microscopy. The embryos were injected with ethanol on the 3rd or 6th day of incubation and observed until days 13, 15, 17 and 21 of development. A decrease was seen in the number of germinal cells generated, together with defects in neuronal migration and the existence of a lower quantity of cells due to a generalised process of cell death.

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Pulmonary pressure-flow curves, hemodynamic and blood gas parameters in West's zone II condition were obtained in nine isolated in situ left lower lobes (LLL) before (condition A), and after 15, 30 and 60 minutes of lobar gelfoam embolization (GE) (condition B) in order to know the natural history of this model. After GE a decrease in PaO2 and lobar O2 were noticed (p less than 0.05).

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In an isolated in situ canine left lower lobe preparation we studied the influence of the distal vascular compartment or venous pressure (PV) on the pressure of arterial vascular segment at zero flow or minimal critical closing pressure (MCCP), while alveolar pressure was held constant and below MCCP. The MCCP-PV curve showed an independent segment when PV was less than 8 mmHg. Above this value (that we define as PV), an slope of 1.

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Non-Hodgkin's lymphomas are frequent in patients with human immune deficiency virus positive antibodies. Exceptional instances of cardiac involvement have been described. We report a case of non-Hodgkin's lymphoma and massive cardiac involvement with antemortem echocardiographic assessment.

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We report our experience with dual chamber pacemakers in 70 patients (mean age 64.5), of whom 42 had AV block and 28 sick sinus syndrome, nine of them associated with AV block. Other cardiac disease coexistent with the conduction disturbance was present in 57% of the patients.

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The migration process of the ventral horn in chick embryo spinal cord cells has been studied between 2.5 and 5 days of incubation (HH-17, HH-26), using the Golgi technique. Two different migratory modes are observed.

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Ten isolated in situ canine left lower lobes (LLL) were studied in West zone II condition. In four dogs (group I) the chest wall surrounding the LLL was surgically removed, in the remaining dogs (group II) this procedure was not done. After basal parameters were obtained, minimal critical pressure (MCCP) was recorded and the influence of increasing alveolar pressure (PA) from o to 20 mmHg was noted.

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In experiments with whole animals infested with a highly malignant strain of Ehrlich ascites tumor cells, serial concentrations of amino acids were determined for host plasma, ascitic fluid, and tumor cells, throughout tumor development. Concentration gradients of glutamine, asparagine, valine, leucine, isoleucine, phenylalanine, tyrosine, histidine, tryptophan, arginine, serine, methionine, and taurine from the host plasma toward the ascitic liquid were established; while on the other hand, concentration gradients from the ascitic liquid toward the plasma were established for glutamate, aspartate, glycine, alanine, proline, and threonine. With the exception of aspartate the concentrations of these amino acids were highest inside the cells.

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Using Golgi's staining technique, we investigated some ganglion cells whose axons do not project out of the retina area. These axons, after following a short trajectory through the optic nerve fiber layer or through the 5th stratum of the inner plexiform layer (IPL), change their direction and end in the inner stratum of the IPL.

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A flux of ornithine from the host tissues to the tumor was deduced from the concentrations of ornithine in plasma, ascitic liquid, liver and tumor cells during tumor growth. The activities of arginase and ornithine decarboxylase in both liver and tumor cells confirmed this proposed ornithine supply. Moreover, "in vitro" incubations of tumor cells showed that glutamine could be an additional source of ornithine for tumors.

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A rapid method for the functional reconstruction of amino acid transport from liver plasma-membrane vesicles using the neutral detergent decanoyl-N-glucamide ('MEGA-10') is described. The method is a modification of that previously employed in this laboratory for reconstitution of amino acid transport systems from kidney brush-border membranes [Lynch & McGivan (1987) Biochem. J.

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Phosphate-dependent glutaminase was purified to homogeneity from isolated mitochondria of Ehrlich ascites-tumour cells. The enzyme had an Mr of 135,000 as judged by chromatography on Sephacryl S-300. SDS/polyacrylamide-gel electrophoresis displayed two protein bands, with Mr values of 64,000 and 56,000.

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Using the Golgi staining method with the modification suggested by Colonnier (J. Anat. 98:327, 1964), we have carried out a morphological study of the bipolar cells of the chicken and classified them into various morphological types.

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Using silver impregnation techniques and light microscopy we have described a new type of interneuron in the adult chicken retina. These cells are located in the innermost level of the INL. Expansions arise from the basal portion of the perikaryon and ascend from there, ending in the inner third of the INL.

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Acetate and the long chain free fatty acid palmitate provoked a decrease in the rates of glutamine utilization and glutamate production in Ehrlich ascites tumor cells incubated with 0.5 mM glutamine. There was a cumulative effect with glucose on glutamine metabolism.

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Inorganic phosphate concentrations and the values of energy charge were determined in the cytosolic and mitochondrial compartments of Ehrlich ascites tumour cells incubated with 5 mM glucose and/or 0.5 mM glutamine. The initial value of inorganic phosphate concentration in the cytosolic compartment decreased in the three incubation conditions assayed; this decrease was greater when glucose was present in the incubation medium.

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