The global prevalence ptxP3 lineage of Bordetella pertussis was rare in young children with the co-purified aPV vaccination: a 5 years retrospective study.

Background: The global prevalent ptxP3 strains varies from about 10% to about 50% of circulating B. pertussis population in different areas of China.

Methods: To investigate the difference of vaccination status between different genotypes in the circulating B.

Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number.

The outbreak of coronavirus disease 2019 (COVID-19) has spread across the world and was characterized as a pandemic. To protect medical laboratory personnel from infection, most laboratories inactivate the virus causing COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in clinical samples before testing. However, the effect of inactivation on the detection results remains unknown.

1.7 μm band narrow-linewidth tunable Raman fiber lasers pumped by spectrum-sliced amplified spontaneous emission.

A 1.7 μm band tunable narrow-linewidth Raman fiber laser based on spectrally sliced amplified spontaneous emission (SS-ASE) and multiple filter structures is proposed and experimentally demonstrated. In this scheme, an SS-ASE source is employed as a pump source in order to avoid stimulated Brillouin scattering.

Direct Detection of Erythromycin-Resistant Bordetella pertussis in Clinical Specimens by PCR.

Resistance of Bordetella pertussis to erythromycin has been increasingly reported. We developed an allele-specific PCR method for rapid detection of erythromycin-resistant B. pertussis directly from nasopharyngeal (NP) swab samples submitted for diagnostic PCR.

Differential expression of miRNAs in enterovirus 71-infected cells.

Background: Enterovirus 71 (EV71) is one of the major etiological pathogens of hand, foot and mouth disease (HFMD) and can cause severe cerebral and pulmonary complications and even fatality. MicroRNAs (miRNAs), a class of small non-coding RNA molecules, play an important role in post-transcriptional regulation of gene expression and thereby influencing various physiological and pathological processes. Increasing evidence suggests that miRNAs act as key effector molecules in the complicated pathogen-host interactions.

Cross reactivity of Neisseria meningitidis crgA diagnostic PCR primers with nontypeable haemophilus influenzae.

Background: CrgA based PCR are usually used for diagnosis of Neisseria meningitidis while the crgA gene was observed in the genomes of Haemophilus influenzae. In this study, we aimed to evaluate whether the crgA primers routinely used in the diagnosis of N. meningitidis could cross react with H.

Outbreak of acute respiratory disease caused by human adenovirus type 7 in a military training camp in Shaanxi, China.

Outbreaks of ARD associated with HAdV have been reported in military populations in many countries. Here, we report an ARD outbreak caused by HAdV-7 in a military training camp in Shaanxi Province, China, from February to March of 2012. Epidemic data and samples from the patients were collected, and viral nucleotides from samples and viral isolations were detected and sequenced.