Measurement of curvature and twist of a deformed object using digital holography.

Measurement of curvature and twist is an important aspect in the study of object deformation. In recent years, several methods have been proposed to determine curvature and twist of a deformed object using digital shearography. Here we propose a novel method to determine the curvature and twist of a deformed object using digital holography and a complex phasor.

Beam-splitting cube for fringe-projection, holographic, and shearographic interferometry.

We describe a simple method in which the techniques of fringe-projection, holographic, and shearographic interferometry may be readily realized with a commercial beam-splitting cube, a laser source, and a beam expander. With the use of a computer algorithm, moiré fringes may be derived digitally from the fringes that are projected onto a reference surface and onto an object surface. Successful use of the beam-splitting cube for these optical methods is attributed to the refraction of the two split beams that exit from the two adjacent faces of the cube.

A key claudin extracellular loop domain is critical for epithelial barrier integrity.

Intercellular tight junctions (TJs) regulate epithelial barrier properties. Claudins are major structural constituents of TJs and belong to a large family of tetra-spanning membrane proteins that have two predicted extracellular loops (ELs). Given that claudin-1 is widely expressed in epithelia, we further defined the role of its EL domains in determining TJ function.

Generation of carrier fringes in holography and shearography.

Double-exposure holography and double-exposure shearography are often used together with the carrier fringe technique, which requires additional shifting of the light source in a prescribed manner between exposures. In the holographic carrier fringe technique, difficulty in prescribing a suitable movement of the light source may be alleviated through visualization of the moiré fringes that are reconstructed by slight displacement of two overlaid families of ellipsoids in a holodiagram. Because shearography is the first differential of holography, it is often impractical to perform two successive optical differentiations on the ellipsoids to visualize the shearographic carrier fringes.

Simultaneous detection of airborne aflatoxin, ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography.

An AOZ method, based on high-performance liquid chromatography (HPLC), was optimized on HPLC condition such as mobile phase and wavelength to simultaneously quantify six kinds of mycotoxins [four aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA)]. Conditions for immunoaffinity clean-up, HPLC and photo-derivatization were optimized in this study and successfully applied in assessment of airborne mycotoxins from a poultry house in Dalian, China. Fifty-two air samples were collected with AGI-30 air samplers using pure water as collection media.

Determination of three-dimensional displacement using two-dimensional digital image correlation.

A novel method that uses a two-dimensional (2D) digital image correlation (DIC) based on a single CCD camera to measure three-dimensional (3D) displacement and deformation is proposed. Rigid-body displacement in 3D space consists of both in-plane and out-of-plane components. The presence of an in-plane displacement component results in a shift of the center of the image displacement vector, while the slope of the image displacement vector is related to the out-of-plane displacement component.

Single electron transfer-promoted photocyclization reactions of linked acceptor-polydonor systems: effects of chain length and type on the efficiencies of macrocyclic ring-forming photoreactions of tethered alpha-silyl ether phthalimide substrates.

Results of an investigation, aimed at gaining information about the factors governing the efficiencies of single electron transfer (SET)-promoted photocyclization reactions of linked acceptor-polydonor systems, are described. One set of substrates used in this effort includes alpha-trimethylsilyl ether terminated, polymethylene- and polyethylenoxy-tethered phthalimides and 2,3-naphthalimides. Photocyclization reactions of the polyethylenoxy-linked phthalimides and naphthalimides were observed to take place in higher chemical yields and with larger quantum efficiencies than those of analogs containing polymethylene tethers of near equal length.

Hospitalization due to adverse drug reactions and drug interactions before and after HAART.

Objective: To characterize and compare the rates of adverse drug reactions (ADRs) and interactions on admission in two, one-year periods: pre-highly active antiretroviral therapy (HAART) (phase 1) and post-HAART (phase 2).

Design: Retrospective chart review.

Setting: University-affiliated tertiary care centre.

A study in glycation of a therapeutic recombinant humanized monoclonal antibody: where it is, how it got there, and how it affects charge-based behavior.

The glycated form of a basic recombinant humanized monoclonal antibody (rhuMAb) was separated and quantitated by boronate affinity chromatography using optimized shielding reagents. Characterization on the isolated glycated material by peptide mapping analysis, using liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) sequencing techniques, identified eight reactive lysine primary amine sites. The glycation reaction extent was similar among the various reactive sites, ranging from approximately 1 to 12%, and a single histidine residue separated the most and least reactive sites.

Combined inhaled diesel exhaust particles and allergen exposure alter methylation of T helper genes and IgE production in vivo.

Changes in methylation of CpG sites at the interleukin (IL)-4 and interferon (IFN)-gamma promoters are associated with T helper (Th) 2 polarization in vitro. No previous studies have examined whether air pollution or allergen exposure alters methylation of these two genes in vivo. We hypothesized that diesel exhaust particles (DEP) would induce hypermethylation of the IFN-gamma promoter and hypomethylation of IL-4 in CD4+ T cells among mice sensitized to the fungus allergen Aspergillus fumigatus.

DUBA: a deubiquitinase that regulates type I interferon production.

Production of type I interferon (IFN-I) is a critical host defense triggered by pattern-recognition receptors (PRRs) of the innate immune system. Deubiquitinating enzyme A (DUBA), an ovarian tumor domain-containing deubiquitinating enzyme, was discovered in a small interfering RNA-based screen as a regulator of IFN-I production. Reduction of DUBA augmented the PRR-induced IFN-I response, whereas ectopic expression of DUBA had the converse effect.

Fabrication of a novel pH-sensitive glutaraldehyde cross-linked pectin nanogel for drug delivery.

A novel pH-sensitive nanogel based on pectin cross-linked with glutaraldehyde (PT-GA) was designed and synthesized for drug delivery. Transmission electron microscope observation shows that the nano-sized gel particles exhibit a spherical morphology. The optical absorbance study of nanogel suspension reveals its pH sensitivity.

Antifungal activity of a novel compound from Burkholderia cepacia against plant pathogenic fungi.

Aims: To investigate antifungal activity of a novel compound (named as CF66I provisionally) against plant pathogenic fungi, mainly including Fusarium sp., Colletotrichum lindemuthianum, Rhizoctonia solani, etc.

Methods And Results: Minimal inhibition concentrations (MIC) and minimal fungicidal concentrations (MFC) of CF66I for each fungi were determined using serial broth dilution method.

Temporal phase retrieval from a complex field in digital holographic interferometry.

We describe a novel method of processing complex phasors in digital holographic interferometry (DHI). Unlike the commonly used digital phase subtraction method that operates on the phase itself, the proposed method operates on the complex phasor instead. Two temporal phase retrieval algorithms are developed in which the complex phasor of each pixel is measured and analyzed as a function of time.

[Genetical diagnosis in a family with X-linked hypohidrotic ectodermal dysplasia].

Objective: To identify the mutations of ED1 gene in a family with X-linked hypohidrotic ectodermal dysplasia

Methods: Eight coding exons of ED1 gene of two patients with clinically confirmed X-linked hypohidrotic ectodermal dysplasia, their parents, and 100 unrelated population-matched control were amplified by polymerase chain reaction. The products were further analyzed by direct sequencing.

Results: Two patients with X-linked hypohidrotic ectodermal dysplasia in this pedigree showed a point mutation at nucleotide 1 045 ( A > G) .

The familial risk of acne vulgaris in Chinese Hans - a case-control study.

Background: Acne is a chronic inflammatory disease of the pilosebaceous follicles. Recent studies bring us increasing evidences that hereditary factors play an important but indirect role in acne.

Objective: To investigate the possible role of genetic factors in the pathogenesis of acne vulgaris in Chinese Han ethnic group.

Ala657 and conserved active site residues promote fibroblast activation protein endopeptidase activity via distinct mechanisms of transition state stabilization.

Fibroblast activation protein (FAP) and dipeptidyl peptidase-4 (DPP-4) are highly homologous serine proteases of the prolyl peptidase family and therapeutic targets for cancer and diabetes, respectively. Both proteases display dipeptidyl peptidase activity, but FAP alone has endopeptidase activity. FAP Ala657, which corresponds to DPP-4 Asp663, is important for endopeptidase activity; however, its specific role remains unclear, and it is unknown whether conserved DPP-4 substrate binding residues support FAP endopeptidase activity.

Global and local coordinates in digital image correlation.

Digital image correlation (DIC) is commonly used to measure specimen displacements by correlating an image of a specimen in an undeformed or reference configuration and a second image under load. To establish the correlation between the images, numerical techniques are used to locate an initially square image subset in a reference image. In this process, choosing appropriate coordinates is of fundamental importance to ensure accurate results.

A prospective investigation of outcomes after hospital discharge for endemic, community-acquired methicillin-resistant and -susceptible Staphylococcus aureus skin infection.

Background: Although community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infection has become increasingly common, prospective data on outcomes of patients with skin infection remain poorly defined.

Methods: We prospectively observed a cohort of 201 patients discharged after hospitalization for CA-MRSA infection or community-acquired methicillin-susceptible S. aureus (CA-MSSA) infection.

Synthesis and structure-activity relationship of N-acyl-Gly-, N-acyl-Sar- and N-blocked-boroPro inhibitors of FAP, DPP4, and POP.

The structure-activity relationship of various N-acyl-Gly-, N-acyl-Sar-, and N-blocked-boroPro derivatives against three prolyl peptidases was explored. Several N-acyl-Gly- and N-blocked-boroPro compounds showed low nanomolar inhibitory activity against fibroblast activation protein (FAP) and prolyl oligopeptidase (POP) and selectivity against dipeptidyl peptidase-4 (DPP4). N-Acyl-Sar-boroPro analogs retained selectivity against DPP4 and potent POP inhibitory activity but displayed decreased FAP inhibitory activity.

Discovery and in vitro biosynthesis of haloduracin, a two-component lantibiotic.

Lantibiotics are ribosomally synthesized peptides that undergo posttranslational modifications to their mature, antimicrobial form. They are characterized by the unique amino acids lanthionine and methyllanthionine, introduced by means of dehydration of Ser/Thr residues followed by reaction of the resulting dehydro amino acids with cysteines to form thioether linkages. Two-component lantibiotics use two peptides that are each posttranslationally modified to yield two functionally distinct products that act in synergy to provide bactericidal activity.

Characterization of dynamic microgyroscopes by use of temporal digital image correlation.

The advanced mechanical testing of microelectromechanical systems (MEMS) is necessary to provide feedback of measurements that can help the designer optimize MEMS structures and improve the reliability and stability of MEMS. We describe a digital image correlation (DIC) method for dynamic characterization of MEMS using an optical microscope with a high-speed complementary metaloxide semiconductor-based camera. The mechanical performance of a series of microgyroscopes is tested.