In vivo gene editing of T-cells in lymph nodes for enhanced cancer immunotherapy.

Immune checkpoint blockade (ICB) therapy, while promising for cancer treatment, faces challenges like unexpected side effects and limited objective responses. Here, we develop an in vivo gene-editing strategy for improving ICB cancer therapy in a lastingly effective manner. The approach uses a conductive hydrogel-based electroporation system to enable nucleofection of programmed cell death protein 1 (PD1) targeted CRISPR-Cas9 DNAs into T-cells directly within the lymph nodes, and subsequently produces PD1-deficient T-cells to combat tumor growth, metastasis and recurrence in different melanoma models in mice.

Multifunctional hydrogel electronics for closed-loop antiepileptic treatment.

Closed-loop strategies offer advanced therapeutic potential through intelligent disease management. Here, we develop a hydrogel-based, single-component, organic electronic device for closed-loop neurotherapy. Fabricated out of conductive hydrogels, the device consists of a flexible array of microneedle electrodes, each of which can be individually addressed to perform electrical recording and control chemical release with sophisticated spatiotemporal control, thus pioneering a smart antiseizure therapeutic system by combining electrical and pharmacological interventions.

Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods.

The procedure of anterior cruciate ligament (ACL) allograft preparation can be divided into fresh-frozen method (FF-allograft) or freeze-dried method (FD-allograft). This study aims to biomechanically and histologically compare the graft to bone tunnel integration between the two allografts. In-vitro results indicated that FF-allograft and FD-allograft showed excellent biocompatibility and biomechanics, while FD-allograft showed a denser collagen fiber arrangement than FF-allograft and autograft.

Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar-Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants.

Although solar steam generation strategy is efficient in desalinating seawater, it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic pollutants. Herein, dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co(OH)CO nanorod top layer and a bacterial cellulose/Co(OH)CO nanorod (BCH) bottom layer. Crucially, the hydrogen bonding networks inside the membrane can be tuned by the rich surface -OH groups of the bacterial cellulose and Co(OH)CO as well as the ions and radicals in situ generated during the catalysis process.

Association between glycemia and multi-vessel lesion in participants undergoing coronary angiography: a cross-sectional study.

Background: This study aims to elucidate the association between glycemia and the occurrence of multi-vessel lesions in participants undergoing coronary angiography.

Methods: We analyzed 2,533 patients with coronary artery disease who underwent coronary angiography. Of these, 1,973 patients, identified by the endpoint of multi-vessel lesions, were examined using univariate and multivariate logistic regression analyses to determine the relationship between glycemia levels and multi-vessel lesion occurrence.

Gradient Lithium Ion Regulation Current Collectors for High-Performance and Dendrite-Free Li Metal Batteries.

Lithium metal anode has attracted wide attention due to its ultrahigh theoretical specific capacity, lowest reduction potential, and low density. However, uncontrollable dendritic growth and volume change caused by uneven Li deposition still seriously hinder the large-scale commercial application of lithium metal batteries, even causing serious battery explosions and other safety problems. Hence, gold nanoparticles with a gradient distribution anchored on 3D carbon fiber paper (CP) current collectors followed by the encapsulation of polydopamine (PDA) (CP/Au/PDA) are constructed for stable and dendrite-free Li metal anodes for the first time.

PGD 2 /DP1 axis promotes liver regeneration by secreting Wnt2 in KCs in mice.

Background And Aims: The liver possesses a remarkable regenerative capacity in response to injuries or viral infections. Various growth factors and cytokines are involved in regulating liver regeneration. Prostaglandin D 2 , a pro-resolution lipid mediator, is the most abundant hepatic prostanoid.

Transdermal microarrayed electroporation for enhanced cancer immunotherapy based on DNA vaccination.

Despite the tremendous clinical potential of nucleic acid-based vaccines, their efficacy to induce therapeutic immune response has been limited by the lack of efficient local gene delivery techniques in the human body. In this study, we develop a hydrogel-based organic electronic device (μEPO) for both transdermal delivery of nucleic acids and in vivo microarrayed cell electroporation, which is specifically oriented toward one-step transfection of DNAs in subcutaneous antigen-presenting cells (APCs) for cancer immunotherapy. The μEPO device contains an array of microneedle-shaped electrodes with pre-encapsulated dry DNAs.

Studies on the Biomimetic Synthesis of Marine Ladder Polyethers via Endo-Selective Epoxide-to-Epoxonium Ring-Opening Cascades.

Marine ladder polyethers have attracted the attention of chemists and biologists because of their potent biological activities. Synthetic chemists have attempted to construct their polyether frameworks by epoxide ring-opening cascades, as Nakanishi hypothesis describes. However, Baldwin's rules of ring closure state that exo-selective intramolecular cyclization of epoxy alcohols is preferred over endo-selective cyclization.

Redox-neutral α-functionalization of pyrrolidines: facile access to α-aryl-substituted pyrrolidines.

α-Aryl-substituted pyrrolidine moiety is found in many natural alkaloids. Starting from pyrrolidine, we were able to synthesize α-aryl-substituted pyrrolidines in one step using quinone monoacetal as the oxidizing agent and DABCO as the base. We also discovered the reaction condition needed to efficiently remove the -aryl moiety from the α-arylated product.

Well-designed lamellar reduced graphene oxide-based foam for high-performance solar-driven water purification.

Although solar steam generation is promising for seawater desalination, it is less effective in purifying wastewater with both salt/heavy metal ions and organic contaminants. It is thus imperative to develop multifunctional integrated solar-driven water purification systems with high solar-thermal evaporation and photocatalytic degradation efficiencies. Herein, a lamellar reduced graphene oxide (L-RGO) foam with the vertical lamellar structure is fabricated by bidirectional-freezing, lyophilization, and slight chemical reduction for water purification.

Simultaneous Solar-Thermal Desalination and Catalytic Degradation of Wastewater Containing Both Salt Ions and Organic Contaminants.

Although solar steam generation is promising in generating clean water by desalinating seawater, it is powerless to totally degrade organic contaminants in the seawater. Herein, solar steam generation and catalytic degradation are integrated to generate clean water by simultaneous solar-driven desalination and catalytic degradation of wastewater containing both salt ions and organic contaminants. Stepwise decoration of three-dimensional nickel foam with polypyrrole, reduced graphene oxide (RGO), and cobalt phosphate is realized to obtain polypyrrole/RGO/cobalt phosphate/nickel foam (PGCN) hybrids for solar-driven desalination and catalytic degradation of wastewater containing antibiotics and salt ions.

Hydrothermally Modified 3D Porous Loofah Sponges with MoS Sheets and Carbon Particles for Efficient Solar Steam Generation and Seawater Desalination.

Although the emerging interfacial solar steam generation technology is sustainable and eco-friendly for generating clean water by desalinating seawater and purifying wastewaters, salt deposition on the evaporation surface during solar-driven evaporation severely degrades the purification performances and adversely affect the long-term performance stability of solar steam generation devices. Herein, to construct solar steam generators for efficient solar steam generation and seawater desalination, three-dimensional (3D) natural loofah sponges with both macropores of the sponge and microchannels of the loofah fibers are hydrothermally decorated with molybdenum disulfide (MoS) sheets and carbon particles. Benefiting from fast upward transport of water, rapid steam extraction, and effective salt-resistant capacity, the 3D hydrothermally decorated loofah sponge with MoS sheets and carbon particles (HLMC) with an exposed height of 4 cm can not only obtain heat by its top surface under the downward solar light irradiation based on the solar-thermal energy conversion but also gain environmental energy by its porous sidewall surface, achieving a competitive water evaporation rate of 3.

Prognostic significance of controlling nutritional status score for patients with gastric cancer: A systematic review and meta‑analysis.

There is a need to identify potentially useful biomarker(s) for the prediction of prognostic outcomes in patients diagnosed with gastric cancer. This meta-analysis provided updated evidence on the association of controlling nutritional status (CONUT) score with survival and other clinicopathological outcomes in patients with gastric cancer. PubMed and Scopus databases were systematically searched.

The value of different involvement patterns of the knee "synovio-entheseal complex" in the differential diagnosis of spondyloarthritis, rheumatoid arthritis, and osteoarthritis: an MRI-based study.

Objectives: To explore the different involvement patterns of the knee "synovio-entheseal complex (SEC)" on MRI in patients with spondyloarthritis (SPA), rheumatoid arthritis (RA), and osteoarthritis (OA).

Methods: This study retrospectively included 120 patients (male:female, 55:65) with a mean age of 39.20 years diagnosed with SPA (n = 40), RA (n = 40), and OA (n = 40) at the First Central Hospital of Tianjin between January 2020 and May 2022.

Fabrication of a novel polyurethane foam-alginate-zeolite hydrogel and subsequent KSND bacteria encapsulation: evidence of accelerated biofilm colonization and enhanced nitrogen removal efficiency.

Biofilms are used widely to remove nitrogen from wastewater; however, most biofilm carriers (i.e. polyurethane foam, PUF) are hydrophobic organic materials with millimetre-scale apertures, ineffective attachment, and unstable colonization of microorganisms.

Omnidirectionally irradiated three-dimensional molybdenum disulfide decorated hydrothermal pinecone evaporator for solar-thermal evaporation and photocatalytic degradation of wastewaters.

Although most solar steam generation devices are effective in desalinating seawater and purifying wastewaters with heavy metal ions, they are ineffective in degrading organic pollutants from wastewaters. Herein, we design novel solar-driven water purification devices by decoration of three-dimensional pinecones with MoS nanoflowers via a one-step hydrothermal synthesis for generating clean water. The vertically arrayed channels in the central rachis and the unique helically arranged scales of the hydrothermal pinecone can not only transfer bulk water upward to the evaporation surface, but also absorb more solar light from different incident angles for solar-thermal evaporation and photodegradation of wastewaters under omnidirectional irradiations.

In-Situ Constructing A Heterogeneous Layer on Lithium Metal Anodes for Dendrite-Free Lithium Deposition and High Li-ion Flux.

Constructing efficient artificial solid electrolyte interface (SEI) film is extremely vital for the practical application of lithium metal batteries. Herein, a dense artificial SEI film, in which lithiophilic Zn/Li Zn are uniformly but nonconsecutively dispersed in the consecutive Li -conductors of Li SiO , Li O and LiOH, is constructed via the in situ reaction of layered zinc silicate nanosheets and Li. The consecutive Li -conductors can promote the desolvation process of solvated-Li and regulate the transfer of lithium ions.

Peripheral enthesitis assessed by whole-body MRI in axial spondyloarthritis: Distribution and diagnostic value.

Objective: To determine the distribution and diagnostic value of peripheral enthesitis detected by whole-body MRI (WBMRI) in axial spondyloarthritis (axSpA) diagnosis, and to determine the value of the peripheral enthesitis score in axSpA assessment.

Methods: Sixty axSpA patients [mean age of 33.2 (24.

Baseline Severity of Sacroiliitis and Extensive Fat Metaplasia Predicts the Progression of Backfill at the Sacroiliac Joint in Patients With Axial Spondyloarthritis.

Objectives: Fat metaplasia in an erosion cavity, also known as backfill, is an essential intermediary in new bone formation in axial spondyloarthritis (axSpA) patients; however, the predictors of backfill progression are unknown. This longitudinal study aimed to assess the predictors of backfill progression in axSpA patients on magnetic resonance imaging (MRI).

Methods: Clinical and MRI data were collected at baseline and follow-up in 52 axSpA patients.

Evaluation of active inflammation, chronic structural damage, and response to treatment of sacroiliitis in axial spondyloarthritis using the Spondyloarthritis research consortium of Canada scoring system.

Background: Axial spondyloarthritis (axSpA) is a chronic inflammatory rheumatic disease affecting the spine and sacroiliac joints. To investigate whether there are differences in inflammatory and chronic structural damages, as assessed by a semiquantitative MRI scoring method, between non-radiographic axial spondyloarthritis (nr-axSpA) and ankylosing spondylitis (AS) patients with active inflammation at baseline, and to evaluate the treatment response in these patients after 3 months of tumor necrosis factor-alpha (TNF-α) inhibitor treatment.

Methods: Fifty-eight axSpA patients with active inflammation were included in the study.