Non-point source (NPS) pollution directly threatens river water quality, constrains sustainable economic development, and poses hazards to human health. Comprehension of the impact factors on NPS pollution is essential for scientific river water quality management. Despite the landscape pattern being considered to have a significant impact on NPS pollution, the driving mechanism of landscape patterns on NPS pollution remains unclear.
View Article and Find Full Text PDFGlobal irrigation areas face the contradictory challenges of controlling nitrate inputs and ensuring food-safe production. To prevent and control nitrate pollution in irrigation areas, the study using the Yellow River basin (Ningxia section) of China as a case study, employed nitrogen and oxygen dual isotope tracing and extensive field investigations to analyze the sources, fate, and influencing factors of nitrate in agricultural drainage ditches. The results of source tracing of nitrate showed that annual proportions of nitrate sources entering the Yellow River in the ditches are as follows: for manure & sewage, fertilizer, and natural sources, the ratios are 33%, 35%, and 32% overall.
View Article and Find Full Text PDFCurrently, the comprehensive effect of the landscape pattern on river water quality has been widely studied. However, the interactive influences of landscape type, namely composition (COM) and configuration (CON) on water quality variations, as well as the specific landscape driving types affecting water quality variations under different spatial and seasonal scales remain unclear. To further improve the effectiveness of landscape planning and water quality protection, this study collected monthly water samples from the Fengyu River Watershed in southwestern China from 2018 to 2021, the Biota-Environment Matching Analysis (Bioenv) was used to identify key metrics representing landscape COM and CON, respectively.
View Article and Find Full Text PDFFront Plant Sci
December 2023
Salt stress detrimentally impacts plant growth, imperiling crop yield and food quality. Ameliorating plant resilience and productivity in saline environments is critical for global food security. Here, we report the positive effect of (Spirulina) on plant growth and salt tolerance in and sweet sorghum.
View Article and Find Full Text PDFCurrently, limited information is available in the literature regarding the relationships between PROC mutations and clinical features in Chinese individuals. We aimed to characterize severe congenital Protein C deficiency in 22 unrelated Chinese families in a tertiary hospital by analyzing its clinical manifestation, associated risk factors, and gene mutations. We measured protein C activity and antigen levels for all participants, screened them for mutations in the PROC gene, and analyzed the clinical features of each family to identify commonalities and differences.
View Article and Find Full Text PDFConsensus has emerged that landscape pattern evolution significantly impacts the river environment. However, there remains unclear how the landscape pattern evolves possible to achieve a balance between land resource use and water conservation. Thus, simulating future landscape patterns under different scenarios to predict river eutrophication level is critical to propose targeted landscape planning programs and alleviate river water quality degradation.
View Article and Find Full Text PDFWidespread attention has been given to understanding the effect of the landscape pattern on river water quality. However, which spatial scale (riparian zone versus sub-basin) has the greater impact on water quality has long been controversial, since the key metrics that affect water quality varied with spatial scale. Thus, quantifying the spatial scale effects of key landscape metrics on water quality is critical to clarifying which scale of landscape pattern is more conducive to water quality conservation.
View Article and Find Full Text PDFZhonghua Yi Xue Yi Chuan Xue Za Zhi
July 2022
Objective: To explore the molecular pathogenesis of hereditary protein C (PC) deficiency due to a p.Gly86Asp variant of the PROC gene through in vitro expression experiment.
Methods: Wild type and Gly86Asp mutant expression plasmids of PC were constructed and respectively transfected into HEK 293FT cells.
Background: The study aimed to investigate the effect of low-frequency electrical stimulation (LFES) on disuse muscle atrophy and its mechanism in a rabbit model of knee extension contracture.
Methods: This study involved two experiments. In the time-point experiment, 24 rabbits were randomly divided into 4 groups: Control 1 (Ctrl1 group), immobilization for 2 weeks (I-2 group), immobilization for 4 weeks (I-4 group), and immobilization for 6 weeks (I-6 group).
Sucrose is the central unit of carbon and energy in plants. Active intercellular transport of sucrose is mediated by sucrose transporters (SUTs), genes for which have been found in the genomes of all land plants. However, they have only been assigned functions in angiosperm species.
View Article and Find Full Text PDFThe purpose of this study was to determine the preventive effect of ultrashort wave diathermy on immobilization-induced myogenic contracture and to explore its underlying mechanisms. Forty-two rabbits were randomly assigned into control (Group C), immobilization (Group I, which was further divided into one week, Group I-1; two weeks, Group I-2; and four weeks, Group I-4, subgroups by the length of immobilization) and ultrashort wave prevention (Group U, which was further divided into one week, Group U-1; two weeks, Group U-2; and four weeks, Group U-4, by time of treatment) groups. Intervention effects were assessed by evaluating rectus femoris cross-sectional area (CSA), knee range of motion, and the protein levels for myogenic differentiation (MyoD) and muscle atrophy F-box (MAFbx-1) in the rectus femoris.
View Article and Find Full Text PDFBacterioplankton communities in rivers are strongly influenced by the surrounding landscape, yet the relationships between land use and bacterioplankton communities at multi-spatial scales and the mechanisms that shape bacterioplankton communities remain unclear. Here, we collected surface water samples from 14 tributaries of the Yuan River, a secondary tributary of the Yangtze River, which has been heavily impacted by human activities. We characterized the bacterioplankton communities by high-throughput sequencing techniques, and managed to identify the mechanisms governing bacterioplankton community assembly.
View Article and Find Full Text PDFBlood Coagul Fibrinolysis
December 2021
To explore the pathogenesis of protein C (PC) deficiency in two independent families by mutations detection and bioinformatics analysis. The PC activity (PC:A) and PC antigen (PC:Ag) were detected by chromogenic substrate and ELISA, respectively. The PROC sequencing was performed to identify the mutational sites.
View Article and Find Full Text PDFPlant acclimation and stress responses depend on the dynamic optimization of carbon balance between source and sink organs. This optimization also applies to the leaf export rate of photosynthetically produced sugars. So far, investigations into the molecular mechanisms of how the rate is controlled have focused on sugar transporters responsible for loading sucrose into the phloem sieve element-companion cell complex of leaf veins.
View Article and Find Full Text PDFObjective: This article aims to analyze the phenotype and genotype of an inherited dysfibrinogenemia pedigree associated with a heterozygous mutation in the gene, and to investigate the pathogenesis of this disease.
Clinical Presentation: The proband of interest is a 29-year-old woman. She was in her 37 weeks of gestation.
Blood Coagul Fibrinolysis
January 2021
o explore the phenotype and genotype of a hereditary antithrombin deficient Chinese family. Functional and molecular analysis of the proband and his family members was performed. Online bioinformatics software was used to predict the pathogenicity of the novel mutation.
View Article and Find Full Text PDFObjective: The purpose of this study was to examine the intervention effect of radial extracorporeal shock wave combined with ultrashort wave diathermy on immobilization-induced fibrosis and contracture of muscle.
Design: The groups included male rabbits for the group (control group). To cause joint contracture, rabbits underwent plaster fixation of a left knee joint at full extension.
Blood Coagul Fibrinolysis
December 2020
: The aim of this study was to elucidate the molecular defects in a Chinese family with dysfibrinogenemia. The fibrinogen activity was measured by the one-stage clotting method. The fibrinogen antigen was measured with immunoturbidimetry.
View Article and Find Full Text PDFAntithrombin (AT) is one of the physiological anticoagulants that are mainly synthesized in the liver. As a protease inhibitor belonging to the serpin superfamily, AT is able to inactivate thrombin and inhibit activated coagulation factors IX, X, XI, and XII (FIXa, FXa, FXIa, and FXIIa).1 Moreover, it has been found that AT can inhibit activated FVII (FVIIa) by accelerating dissociation of FVIIa-tissue factor complex and preventing it from recombining.
View Article and Find Full Text PDFThe plant hormone auxin serves as central regulator of growth and development. Auxin transporters in the plasma membrane are assumed to define tissue-level patterns of auxin distribution [1, 2]. However, auxin is small enough to diffuse through the plasmodesmata that connect neighboring cells [3], presenting an alternative pathway, whose contribution to auxin transport remained largely unexplored [4].
View Article and Find Full Text PDFProc Natl Acad Sci U S A
March 2020
All multicellular organisms keep a balance between sink and source activities by controlling nutrient transport at strategic positions. In most plants, photosynthetically produced sucrose is the predominant carbon and energy source, whose transport from leaves to carbon sink organs depends on sucrose transporters. In the model plant , transport of sucrose into the phloem vascular tissue by SUCROSE TRANSPORTER 2 (SUC2) sets the rate of carbon export from source leaves, just like the SUC2 homologs of most crop plants.
View Article and Find Full Text PDFMethods Mol Biol
March 2020
Sugars that are produced by photosynthesis in the leaves are transported in the phloem to heterotrophic sink tissues like roots, fruit, or flowers. Since sugars inside the highly specialized cells of the phloem move by bulk flow, it is the loading and unloading of sugars that determines the rates of allocation between organs. Here, a method is described for the relative quantification of sugars that are loaded into the phloem in leaves.
View Article and Find Full Text PDFAmphioxus, a cephalochordate, is an ideal animal in which to address questions about the evolution of regenerative ability and the mechanisms behind the invertebrate to vertebrate transition in chordates. However, the cellular and molecular basis of tail regeneration in amphioxus remains largely ill-defined. We confirmed that the tail regeneration of amphioxus is a vertebrate-like epimorphosis process.
View Article and Find Full Text PDFSuc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various environmental stimuli in the leaves of five dicots (Arabidopsis [], soybean [], potato [], tomato [], and poplar [ spp.
View Article and Find Full Text PDFIn the sieve elements (SEs) of the phloem, carbohydrates are transported throughout the whole plant from their site of production to sites of consumption or storage. SE structure, especially of the pore-rich end walls, has a direct effect on translocation efficiency. Differences in pore size and other features were interpreted as an evolutionary trend towards reduced hydraulic resistance.
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