Effect of no-touch versus conventional technique on arteriovenous fistula outcomes: a randomised controlled trial.

Background: Autologous arteriovenous fistulae (AVFs) are the best type of vascular access in patients with kidney failure. However, the conventional technique has a high failure rate. We performed a randomised controlled trial to investigate whether the no-touch technique has a higher maturation and patency rate than that of the conventional technique for creating AVFs.

A self-powered and drug-free diabetic wound healing patch breaking hyperglycemia and low HO limitations and precisely sterilizing driven by electricity.

Accelerating diabetes-related chronic wound healing is a long-sought-after goal in diabetes management. However, therapeutic strategies based on antibiotics or catalysts still face great challenges to break the limitations of antimicrobial resistance, low HO and the blocking effect of bacterial biofilms on antibiotic/catalyst penetration. Herein, we reported a glucose biofuel cell-powered and drug-free antibacterial patch, which consisted of an MAF-7 protected glucose oxidase/horseradish peroxidase anode and a horseradish peroxidase cathode, for treating diabetic wounds.

Damage-Free and Time-Saving Platform Integrated by a Flow Membrane Separation Device and a Dual-Target Biofuel Cell-Based Biosensor for Continuous Sorting and Detection of Exosomes and Host Cells in Human Serum.

The growth relationship between exosomes (EXOs) and the host cells is highly desired for tumor evaluations, which puts forward high demand on the accurate and convenient acquisition of their individual quantitative information. However, the tedious and destructive separation process and the requirement of dual-channel detection make it become an extremely challenging task. Herein, we integrated an enzymatic biofuel cell (EBFC)-powered biosensor with a flow cell-supported membrane separation device (FMSC) to develop a continuous separation and detection platform for EXOs and host cancer cells in human serum.

A protein scaffold enables hydrogen evolution for a Ni-bisdiphosphine complex.

An artificial metalloenzyme acting as a functional biomimic of hydrogenase enzymes was activated by assembly covalent attachment of the molecular complex, [Ni(PNP)], within a structured protein scaffold. Electrocatalytic H production was observed from pH 3.0 to 10.

Prognostic Impact of Metabolic Syndrome in Patients With Heart Failure: A Meta-Analysis of Observational Studies.

The metabolic syndrome (MS) is significantly associated with the risk of incident heart failure (HF). However, there are still great controversies about the impact of MS on the prognosis in patients with established HF. This meta-analysis aimed to ascertain the effect of MS on the prognosis in patients with HF.

Harnessing the power of directed evolution to improve genome editing systems.

The recent development of genome editing systems, such as zinc-finger nucleases, transcription activator-like effectors, CRISPR-Cas nucleases, and base editors has enabled the unprecedented capability to engineer the genomes of living cells. The ever-increasing demand for genome editors with improved accuracy, activity, and functionality has stimulated significant efforts to further engineer the genome editing systems. Directed evolution represents a promising strategy to improve the existing genome editing systems and enable new editing functions.

Label-free detection of exosomes based on ssDNA-modulated oxidase-mimicking activity of CuCoO nanorods.

A label-free method for exosome detection was proposed. It is based on the target-responsive controllability of oxidase-like activity of Cu/Co bimetallic metal-organic frameworks (CuCoO nanorods). In the absence of exosomes, the oxidase-like activity was inhibited due to the adsorption of CD63 aptamer onto nanorods' surface.

Novel M -selective, G -biased agonists of muscarinic acetylcholine receptors.

Background And Purpose: More than 30% of currently marketed medications act via GPCRs. Thus, GPCRs represent one of the most important pharmacotherapeutic targets. In contrast to traditional agonists activating multiple signalling pathways, agonists activating a single signalling pathway represent a new generation of drugs with increased specificity and fewer adverse effects.

Extending mechanochemical porphyrin synthesis to bulkier aromatics: tetramesitylporphyrin.

Aldehydes with bulky substituents in the -positions have been historically difficult in porphyrin synthesis, presumably owing to steric hindrance around the reactive site. We have used mechanochemistry for the simple, room-temperature synthesis of tetra--substituted porphyrins. In the present study, mesitaldehyde undergoes acid-catalyzed mechanochemical condensation with pyrrole to give -tetrakis[2,4,6-(trimethyl)phenyl]porphyrin (TMP) after oxidation in solution.