Synbiotic Effects of Lacticaseibacillus paracasei K56 and Prebiotics on the Intestinal Microecology of Children with Obesity.

Lacticaseibacillus paracasei K56 (L. paracasei K56) is a probiotic with weight-loss effects. However, symbiosis research on the combined effects of Lacticaseibacillus paracasei K56 and prebiotics is lacking.

Bifidobacterium longum subsp. infantis YLGB-1496-Toxicological evaluation.

Bifidobacterium infantis YLGB-1496, originally isolated from breast milk from a Taiwanese mother, is under study for use as a probiotic. As part of safety assessment, an Ames, in vivo mouse micronucleus, and in vivo mouse spermatocyte chromosome aberration assay were conducted along with a 13-week oral rat toxicity study. B.

Quantitative PCR Assays for the Strain-Specific Identification and Enumeration of Probiotic Strain X253.

Probiotics are universally recognized for their health benefits, despite the fact that their effects depend on the strain. Identification and enumeration of probiotic strains are required prior to evaluating their effectiveness. X253 is a potential probiotic strain with antioxidant capacity.

Abundance and Niche Differentiation of Comammox in the Sludges of Wastewater Treatment Plants That Use the Anaerobic-Anoxic-Aerobic Process.

Complete ammonia oxidizers (comammox), which directly oxidize ammonia to nitrate, were recently identified and found to be ubiquitous in artificial systems. Research on the abundance and niche differentiation of comammox in the sludges of wastewater treatment plants (WWTPs) would be useful for improving the nitrogen removal efficiency of WWTPs. Here, we investigated the relative abundance and diversity of comammox in fifteen sludges of five WWTPs that use the anaerobic−anoxic−aerobic process in Jinan, China, via quantitative polymerase chain reaction and high-throughput sequencing of the 16S rRNA gene and ammonia monooxygenase gene.

Exogenous nitrogen from riverine exports promotes soil methane production in saltmarshes in China.

Methane emissions from saltmarshes can potentially promote climate warming. Soil methane production is positively correlated with methane emissions from saltmarshes. Understanding the factors influencing soil methane production will improve the prediction of methane emissions, but an investigation of these factors has not been conducted in saltmarshes in China.

Peptide identification of hepatocyte growth-promoting factor and its function in cytoprotection and promotion of liver cell proliferation through the JAK2/STAT3/c-MYC pathway.

Hepatocyte growth-promoting factor (pHGF) has a significant effect in promoting liver cell proliferation and restoring liver function. In this study, 815 short peptides of pHGF were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), of which 574 short peptides were assigned to 152 proteins related to hemoglobin subunits and some catalytic enzymes, indicating that pHGF might participate in the oxidation-reduction process by regulating reactive oxygen species (ROS) production. Proteomic analysis was used to identify the differentially expressed proteins (DEPs) in SMMC-7721 and L-02 cells after pHGF treatment, which suggested that pHGF had a significant impact on the JAK-STAT signaling pathway and the cell cycle of liver cells.

Ammonium promoting methane oxidation by stimulating the Type Ia methane-oxidizing bacteria in tidal flat sediments of the Yangtze River estuary.

Estuary and coastal environments have essential ecosystem functions in greenhouse gas sinks and removal of nitrogen pollution. Methane-oxidizing bacteria (MOB) and ammonia-oxidizing bacteria (AOB) communities play critical functions in the estuary's tidal flat sediments. Therefore, the effects of ammonium on MOB communities and methane on AOB communities need to be further explained.

Genome analysis of plant growth-promoting rhizobacterium Pseudomonas chlororaphis subsp. aurantiaca JD37 and insights from comparasion of genomics with three Pseudomonas strains.

Pseudomonas chlororaphis subsp. aurantiaca strain JD37 is a plant growth-promoting rhizobacterium (PGPR), which has important biotechnological features such as plant growth promotion, rhizosphere colonization and biocontrol activities. In present study, the genome sequence of JD37 was obtained and comparative genomic analysis were performed to explore unique features of the JD37 genome and its relationship with other Pseudomonas PGPR: P.

Aggressive behavior variation and experience effects in three families of juvenile Chinese mitten crab (Eriocheir sinensis).

To assess how variable is the aggressive behavior among families (A, B, and C) and the experience effect of fighting among juvenile Chinese mitten crab (Eriocheir sinensis), we performed a total of 36 pairs of intrafamily and interfamily contests between three families of Eriocheir sinensis, qualifying and quantifying their aggressive acts and 13 pairs of winners within family and between family A and B. A table of aggression intensity was established, ranging from 1 (chasing) to 4 (intense combat). Crabs of intrafamily association performed more aggressive acts of shorter duration than interfamily, family B was more aggressive than those from families A and C: family C was the least aggressive, which is also the most morphologically distinct strain (a new strain with a red carapace).

Microbial Communities and Diversities in Mudflat Sediments Analyzed Using a Modified Metatranscriptomic Method.

Intertidal mudflats are land-sea interaction areas and play important roles in global nutrient cycles. However, a comprehensive understanding of microbial communities in these mudflats remains elusive. In this study, mudflat sediment samples from the Dongtan wetland of Chongming Island, the largest alluvial island in the world, were collected.

Expression and significance of CHIP in canine mammary gland tumors.

CHIP (Carboxy terminus of Hsc70 Interacting Protein) is an E3 ubiquitin ligase that can induce ubiquitination and degradation of several oncogenic proteins. The expression of CHIP is frequently lower in human breast cancer than in normal breast tissue. However, the expression and role of CHIP in the canine mammary gland tumor (CMGT) remain unclear.

Annexin A2 is involved in the production of classical swine fever virus infectious particles.

Annexin A2 (ANXA2) is an important host factor regulating several key processes in many viruses. To evaluate the potential involvement of ANXA2 in the life cycle of classical swine fever virus (CSFV), an RNA interference (RNAi) approach was utilized. Knockdown of ANXA2 did not impair CSFV RNA replication but significantly reduced CSFV production.

Complete genome sequence of the plant growth-promoting rhizobacterium Pseudomonas aurantiaca strain JD37.

Pseudomonas aurantiaca Strain JD37, a Gram-negative bacterium isolated from potato rhizosphere soil (Shanghai, China), is a plant growth-promoting rhizobacterium. The JD37 genome consists of only one chromosome with no plasmids. Its genome contains genes involved plant growth promoting, biological control, and other function.

Characterization of the C-terminal sequence of NS5A necessary for the assembly and production of classical swine fever virus infectious particles.

Recent studies show that classical swine fever virus (CSFV) NS5A is an essential replicase component, but it is not known how NS5A participates in viral particle production. In this study, deletion and substitution mutations were introduced into the C-terminus of CSFV NS5A. The efficiency of Core protein release and extracellular and intracellular infectivity levels were assessed and NS5A-Core interaction was investigated.

Pseudomonas stutzeri strain possessing a self-transmissible TOL-like plasmid degrades phenol and promotes maize growth in contaminated environments.

Phenol is volatile organic pollutant that plants can little degrade. For complete degradation of volatile pollutants, we introduced Pseudomonas stutzeri strain P7 to phenol-contaminated soils. The strain effectively degraded phenol and even promoted plant growth.

Recombination analyses between two strains of porcine reproductive and respiratory syndrome virus in vivo.

Porcine reproductive and respiratory syndrome virus (PRRSV) is characteristic of genetically extensive variation. In this study, five SPF pigs were co-infected with two strains of PRRSV (JXwn06-81c and HB-1/3.9c), and 352 viruses were cloned by plaque assay from the sera of the infected pigs on days 3, 5, 7, 10, 14, 21 postinfection (pi), and the recombinant events between the two viruses were systematically investigated by sequencing the ORF5, ORF3 and Nsp2 genes of each cloned virus and using SimPlot and Genetic Algorithm for Recombination Detection (GARD) analysis.