Spatial transcriptome analysis reveals de novo regeneration of poplar roots.

Propagation through cuttings is a well-established and effective technique for plant multiplication. This study explores the regeneration of poplar roots using spatial transcriptomics to map a detailed developmental trajectory. Mapping of the time-series transcriptome data revealed notable alterations in gene expression during root development, particularly in the activation of cytokinin-responsive genes.

Histological and single-nucleus transcriptome analyses reveal the specialized functions of ligular sclerenchyma cells and key regulators of leaf angle in maize.

Leaf angle (LA) is a crucial factor that affects planting density and yield in maize. However, the regulatory mechanisms underlying LA formation remain largely unknown. In this study, we performed a comparative histological analysis of the ligular region across various maize inbred lines and revealed that LA is significantly influenced by a two-step regulatory process involving initial cell elongation followed by subsequent lignification in the ligular adaxial sclerenchyma cells (SCs).

Does digital infrastructure improve public Health? A quasi-natural experiment based on China's Broadband policy.

This study proposed a scheme for improving people's health from the perspective of digital infrastructure construction. We used the China Family Panel Studies conducted between 2010 and 2020 and the digital infrastructure construction marked by the Broadband China policy between 2014 and 2016 as a quasi-natural experiment. We adopted the multi-time difference-in-differences method to identify the causal relationship between digital infrastructure and people's health.

Foster care experiences during childhood and health during older age: Evidence from China.

Objective: Improving the health of older individuals is the primary approach to dealing with the challenges of an aging population. Experiences during childhood can have a continual influence on individual health conditions. Consequently, understanding whether foster care experiences during childhood influence an individual's health during older age is relevant.

Gender differences in happiness in Chinese society: The role of parental preference for sons over daughters.

Objective: This paper studies the relationship between Chinese parents' preference for sons over daughters in childhood and these children's happiness in adulthood.

Background: Happiness is an important dimension for measuring a society's well-being and a lifelong goal for many Chinese citizens deeply influenced by Confucianism. However, China's relatively low world ranking on happiness is worrisome for Chinese policymakers and prompts the question of whether the concept of "preference for sons" is related to lower adult happiness.

JianPi-QingHua formula attenuates nonalcoholic fatty liver disease by regulating the AMPK/SIRT1/NF-κB pathway in high-fat-diet-fed C57BL/6 mice.

Context: Non-alcoholic fatty liver disease (NAFLD) is a common liver disease, accompanied by liver lipid accumulation and inflammation. JianPi-QingHua formula (JPQH), a Chinese herbal formula, exhibits effects on obesity and T2DM. However, the hepatoprotective effect of JPQH has not been elucidated.

Tandem mass tag-based proteomic profiling revealed potential therapeutic targets and mechanisms of liraglutide for the treatment of impaired glucose tolerance.

Objective: Based on the tandem mass tag (TMT) technique, our study investigated the potential therapeutic targets of Liraglutide (LIRA) on streptozotocin (STZ) induced impaired glucose tolerance (IGT) in rats and discuss the biological mechanism of the drug against IGT.

Methods: 10 rats were randomly selected from 31 male wistar rats of specific pathogen free (SPF) grade as control group and fed with conventional chow, offered the remaining rats a high fat and high sugar (HFSD) diet combined with an intraperitoneal injection of STZ to establish the IGT model, and excluded 2 non-model rats. Specifically, the model rats were randomly divided into Model group (n=10) and LIRA group (n=9).

CD8 T Cell-Associated Gene Signature Correlates With Prognosis Risk and Immunotherapy Response in Patients With Lung Adenocarcinoma.

The presence of infiltrating CD8 T lymphocytes in the tumor microenvironment of lung adenocarcinoma (LUAD) is correlated with improved patient prognosis, but underlying regulatory mechanisms remain unknown. To identify biomarkers to improve early diagnosis and treatment of LUAD, we downloaded 13 immune cell line-associated datasets from the GEO database. We identified CD8 T cell-associated genes weighted correlation network analysis.

Explore the Effect and Target of Liraglutide on Islet Function in Type 2 Diabetic Rats by miRNA Omics Technology.

Purpose: To analyze the effect and potential therapeutic targets of liraglutide in type 2 diabetes through miRNA expression profiling.

Methods: Ten of 30 SPF Wistar rats, males at 4 weeks old, were randomly selected as the control group and given conventional feed, the other rats adopted high-sugar and high-fat diet combined with an intraperitoneal injection of streptozotocin to establish a T2DM model. One unsuccessful rat was excluded, and the remaining rats were randomized to the model and the liraglutide group.

Pretreatment by composting increased the utilization proportion of pig manure biogas digestate and improved the seedling substrate quality.

Anaerobic digestion of organic residues has the potential to significantly contribute to a shift from fossil to renewable energy, while the remaining biogas digestate need to be treated or used for a second time. In this study, the pig manure biogas digestate (PMBD) was evaluated as a potential part of seedling substrates and composting was considered a pretreating method to improve its characters. Composting was carried out firstly in a forced aeration composting system (100 L), in which perlite and sawdust were used as additives in different proportions separately or together.

Probe into the Target and Mechanism of Jianpi Xiaoke Prescription for Treating Type 2 Diabetes Mellitus through miRNA Expression Profiling.

Methods: Ten of the 31 SPF male Wistar rats were randomly taken as the control group; the remaining rats were fed a high-sugar and high-fat diet, combined with Streptozotocin (STZ, 35 mg/kg) that induced a type 2 diabetes model. The model rats were randomly divided into model groups ( = 11) and the JPXK group ( = 10). After 8 weeks of JPXK intervention, we detected the function of islet cells through HE staining and ELISA.

Based on network pharmacology to explore the molecular mechanisms of astragalus membranaceus for treating T2 diabetes mellitus.

Background: Astragalus membranaceus refers to a type of traditional Chinese medicine (TCM) used to treat type 2 diabetes mellitus (T2DM), whereas its molecular mechanism remains unclear. In the presented study, network pharmacology was performed to analyze the molecular mechanism of astragalus membranaceus against T2DM.

Methods: First, we found common targets of astragalus membranaceus and disease, protein-protein interaction (PPI) network was built by String, and then key targets were screened from these common targets by topological analysis.

Effect of initial material bulk density and easily-degraded organic matter content on temperature changes during composting of cucumber stalk.

To inactivate the potentially pathogenic microorganisms and safely utilize vegetable waste compost, ultra-high temperatures (>70°C) should be maintained during the composting without having an inhibitory effect on maturity. This study investigated the influence of bulk density (part 1) and easily-degraded organic matter content (EDOMC, part 2) on temperature evolution during vegetable waste composting: Part 1: corn straw with different particle sizes was used to achieve different bulk densities in the composting material (BD1-BD3); Part 2: partial or total substitution of the corn straw by corn starch was carried out to obtain different EDOMC (ED1-ED4). The composting experiments were conducted in a lab-scale reactor (1.

Comparing the effects of three in situ methods on nitrogen loss control, temperature dynamics and maturity during composting of agricultural wastes with a stage of temperatures over 70 °C.

The study investigated the effects of three in situ methods for controlling nitrogen loss and maturity with different mechanisms: struvite-based addition (KHPO and MgO, MP), woody peat addition (WP) and intermittent aeration (IA), during composting of vegetable waste (cucumber vine) with temperature over 70 °C to inactivate potential viral pathogens. The experiment was conducted in a 200 L pilot-scale composting system, with which temperature and ammonia emission were recorded in real time, and solid samples were collected and analyzed during the process. The results indicated that the methods of MP and IA reduced the total nitrogen loss by 27.