A modified orthotopic left lung transplantation model in rats.

To enhance the operability of the rat orthotopic left lung transplantation model, we implemented several improvements and meticulously detailed the procedure. One hundred and thirty-one healthy male Sprague Dawley rats, weighing between 250 and 300 g, were utilized, with 64 serving as donors, 64 as recipients, and 3 as sham controls. We employed a modified three-cuff technique for the orthotopic left lung transplantation.

Applications of upconversion nanoparticles in cellular optogenetics.

Upconversion-mediated optogenetics is an emerging powerful technique to remotely control and manipulate the deep-tissue protein functions and signaling pathway activation. This technique uses lanthanide upconversion nanoparticles (UCNPs) as light transducers and through near-infrared light to indirectly activate the traditional optogenetic proteins. With the merits of high spatiotemporal resolution and minimal invasiveness, this technique enables cell-type specific manipulation of cellular activities in deep tissues as well as in living animals.

A survey on applications of deep learning in microscopy image analysis.

Advanced microscopy enables us to acquire quantities of time-lapse images to visualize the dynamic characteristics of tissues, cells or molecules. Microscopy images typically vary in signal-to-noise ratios and include a wealth of information which require multiple parameters and time-consuming iterative algorithms for processing. Precise analysis and statistical quantification are often needed for the understanding of the biological mechanisms underlying these dynamic image sequences, which has become a big challenge in the field.

Downregulated Long Noncoding RNA PART1 Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer.

Objective: In this study, we aimed to clarify the effects of long noncoding ribonucleic acid prostrate androgen-regulated transcript-1 on bladder cancer cell proliferation and apoptosis.

Methods: Microarrays were implemented to investigate the long noncoding ribonucleic acid expression profiles in bladder cancer tissue (N = 9) and in noncancer bladder tissue (N = 5). Relative prostrate androgen-regulated transcript-1 expression levels in tissue samples or cell lines were detected by real-time quantitative reverse transcription-polymerase chain reaction.

Comparative studies on amphotericin B nanosuspensions prepared by a high pressure homogenization method and an antisolvent precipitation method.

Amphotericin B (AmB) is a widely used polyene antifungal agent; however, its poor solubility limits its clinical application. In this study, AmB nanosuspensions were prepared by a high pressure homogenization method (AmB-HPH) and an antisolvent precipitation method (AmB-AP) to improve the drug solubility. To reveal the distinct influences of these two different preparation methods, systematic comparisons of particle size, crystalline state, wettability, in vitro dissolution and in vivo pharmacokinetics on the properties of AmB-HPH and AmB-AP were performed.

Hyaluronic-acid-modified lipid-polymer hybrid nanoparticles as an efficient ocular delivery platform for moxifloxacin hydrochloride.

To improve the ocular bioavailability of the strongly hydrophilic moxifloxacin hydrochloride, hyaluronic-acid-modified lipid-polymer hybrid nanoparticles (HA-LCS-NPs) were designed and characterized. An in vivo precorneal retention study in rabbits showed that the mean residence time (MRT) and area under the curve (AUC) of HA-LCS-NPs were up to 6.74-fold and 4.