Publications by authors named "Qiuping Gao"
Purpose: To investigate the expression of miR-614 in serum of gastric cancer patients and its effect on invasion and proliferation of gastric cancer cell line HGC-27.
Methods: Thirty patients with gastric cancer from May 2016 to November 2018 comprised the research group, and 30 healthy people undergoing physical examination in the same period comprised the control group. The expression of miR-614 in tissues and miR-614 in HGC-27 cell line was detected by qRT-PCR, miR-614-mimics was transfected into HGC-27, while miR-614-mimics group, blank control group and negative control group were established respectively.
Background: Real-time reverse transcription-PCR (rRT-PCR) has been the most effective and widely implemented diagnostic technology since the beginning of the COVID-19 pandemic. However, fuzzy rRT-PCR readouts with high Ct values are frequently encountered, resulting in uncertainty in diagnosis.
Methods: A Specific Enhancer for PCR-amplified Nucleic Acid (SENA) was developed based on the Cas12a trans-cleavage activity, which is specifically triggered by the rRT-PCR amplicons of the SARS-CoV-2 Orf1ab (O) and N fragments.
Objectives: This study aimed to evaluate the potential role of hydrogen in rats after cerebral ischemic/reperfusion (I/R) injury.
Materials And Methods: The experimental samples were composed of sham group, model group of rats that received middle cerebral artery occlusion (MCAO) for 2 hr followed by reperfusion for 24 hr, and the hydrogen saline group treated by hydro¬gen-rich saline (1 ml/kg) after MCAO. Hydrogen sulfide (H2S), S100-βprotein (S100-β), and neuron-specific enolase (NSE) levels were measured; the levels of malondialdehyde (MDA), reactive oxygen species (ROS), and superoxide dismutase (SOD) were detected; the histologic structure and apoptotic cells of hippocampus were observed; the expressions of cystathionine β-synthase (CBS), nuclear factor erythroid 2-related factor 2 (Nrf2), and hemeoxygenase-1 (HO-1) were measured.
Article Synopsis
- Orthaga olivacea, a pest of camphor trees, has had its complete mitochondrial genome sequenced and analyzed to enhance understanding of related Lepidoptera species.
- The mitochondrial genome spans 15,174 bp and comprises 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region, with a strong bias toward adenine and thymine bases.
- Phylogenetic analysis showed that Orthaga olivacea is closely related to Hypsopygia regina within the Pyralidae family, and variations in initiation and termination codons, as well as tRNA structures, were noted in the study.
Characterization and functional analysis of serpin-28 gene from silkworm, Bombyx mori.
J Invertebr Pathol
November 2018
Article Synopsis
- Serine protease inhibitors (Serpins) are important proteins involved in immune responses, and this study focused on a specific serpin-28 gene in the silkworm, B. mori, which encodes a protein of 354 amino acids.
- The expression of Bmserpin-28 was found to be highest in the fat body and silk gland, especially during the pupal stage, and it significantly increased in response to infections from various pathogens.
- Additionally, when Bmserpin-28 was inhibited through RNA interference, there was a notable increase in antimicrobial peptide gene expression, suggesting its crucial role in the innate immune system of B. mori.
Colleterial glands (CG) present in the body of adult female of Bombyx mori, which can help adhere eggs on the surface of the host plants. Although this organ has been known for centuries, only morphology and its secretions have been studied. Their gene expression profiles and physiological roles remain largely unknown.
View Article and Find Full Text PDF[Anti-tumor activity of components isolated from purple sweet potato polysaccharides].
Zhejiang Da Xue Xue Bao Yi Xue Ban
July 2011
Objective: To isolate and purify components from polysaccharides of purple sweet potato (PPSP) and to test their anti-tumor activity.
Methods: DEAE-Cellulose and CM-Cellulose exchange chromatography were applied to separate components of PPSP. The anti-tumor activities of each component were measured by MTT assay on Hela and HepG(2) cells and their monosaccharide composition were analyzed by TLC chromatography, followed by infrared spectroscopy studies.