[The effects of pronase on drugs concentrations for treatment of Helicobacter pylori infection in gastric tissue].

Objective: To evaluate the effect of pronase on amoxicillin and metronidazole concentrations in gastric tissue.

Methods: C57BL/6 mice were randomly divided into experimental group (n = 70) and control group (n = 70). Amoxicillin (28.

Three aromatic amino acids in gastric juice as potential biomarkers for gastric malignancies.

For screening early-stage gastric malignancies, the existing serum biomarkers have limited sensitivity and specificity. Gastric juice biomarkers are scarce and require further investigation. We divided this study on searching potential biomarkers into four parts: (1) detection of differential fluorescence spectrum and peaks in the gastric juice from patients using fluorescence spectroscopy and HPLC, (2) identification and validation of differential peaks using LC/MS and NMR, (3) quantification of potential biomarkers, and (4) establishment of diagnostic detection.

Low levels of vitamin C in dialysis patients is associated with decreased prealbumin and increased C-reactive protein.

Background: Subclinical inflammation is a common phenomenon in patients on either continuous ambulatory peritoneal dialysis (CAPD) or maintenance hemodialysis (MHD). We hypothesized that vitamin C had anti-inflammation effect because of its electron offering ability. The current study was designed to test the relationship of plasma vitamin C level and some inflammatory markers.

Differential efficacy of clarithromycin in lung versus thigh infection models.

Background: Differences in clarithromycin disposition and the resulting changes in bacterial density were studied using mouse lung and thigh infection models.

Methods: Clarithromycin activity was evaluated against seven Streptococcus pneumoniae isolates with efflux-mediated resistance in both murine lung and thigh infection models. Intrapulmonary disposition of clarithromycin was also studied.

Determination of the in vivo pharmacodynamic profile of cefepime against extended-spectrum-beta-lactamase-producing Escherichia coli at various inocula.

Cefepime was evaluated in vivo against two inoculum sizes of four strains of Escherichia coli that produced extended-spectrum beta-lactamases (ESBLs) in a murine neutropenic thigh infection model to characterize the pharmacodynamic activity of cefepime in the presence of ESBL-producing bacteria and to evaluate if differences in lengths of cefepime exposure are required with various inocula. Three strains possessed a single enzyme each: TEM-10, TEM-12, and TEM-26. The fourth strain possessed two TEM-derived ESBLs and a third uncharacterized enzyme.

[Significance of changes in plasma lipid peroxide levels in critically ill patients after liver transplantation].

Objective: To investigate the relationship between plasma lipid peroxide (LPO) levels and prognosis in critically ill patients after liver transplantation.

Methods: Plasma LPO levels were measured in 18 patients within 21 days after liver transplantation, and their relations to the prognosis were analyzed.

Results: Plasma LPO levels were markedly elevated in patients before state of illness got severe, especially in those with fatal outcome.

[Study of autofluorescence spectrum of dilute gastric juice in the diagnosis of gastric carcinoma].

Objective: To investigate the value of the autofluorescence spectrum of dilute gastric juice in the diagnosis of gastric carcinoma.

Methods: The autofluorescence spectrum of dilute gastric juice was scanned with RF-5000 spectrophotofluorometer when the excitation wavelength was set to 288 nm and the emission wavelength ranged from 300 nm to 800 nm.

Results: We enrolled 251 patients with different gastric diseases including 40 cases with gastric carcinoma in our study.

[Evaluation oxygen free radicals related index before liver transplantation to forejudge prognosis].

Objective: To research the relationship of oxygen free radicals related index before liver transplantation with prognosis.

Methods: Lipid peroxide (LPO), total antioxidant capacity (TAC), nitric oxide (NO) and total bilirubin (T-BIL) in blood from 18 patients were measured before liver transplantation, and the relationship with prognosis was analyzed.

Results: The blood LPO of death group was (17.

Simultaneous determination of ticarcillin and clavulanate in rabbit serum and tissue cage fluid by liquid chromatography.

A gradient elution HPLC method with a wavelength switch technique was developed to simultaneously analyze the beta-lactam ticarcillin and the beta-lactamase inhibitor clavulanate in rabbit serum and tissue cage fluid (TCF). A C18 reversed-phase column with a programmable UV detector changing the wavelength from 218 to 254 nm at 9 min was used for chromatographic separation. The mobile phase consisted of acetonitrile, phosphate buffer and tetrabutylammonium hydrogen sulfate by following a gradient elution program at a flow-rate of 1 ml/min.

[The value of autofluorescence spectrum analysis of gastric juice in diagnosing gastric carcinoma: a preliminary result].

Objective: To reveal the difference of autofluorescence spectrums of gastric juice derived from malignancy and benignancy for screening and diagnosis of gastric carcinoma.

Method: Gastric juice from 202 patients with different gastric diseases were collected, then detected their autofluorescence spectrums (the excitation wavelength is 288 nm, whereas the range of emission wavelength is 300 - 800 nm) after diluted by 1:10. The diagnostic model for gastric cancer was made by Classification and Regression Trees V2.