Identification and Validation of Garlic () Metabolites as Quorum Sensing Inhibitors of Targeting the PlcR Receptor: An and Study.

This study aimed to investigate the influence of garlic metabolites on the quorum sensing (QS) of , a foodborne pathogen that controls its main virulence factor through QS. The QS signal receptor PlcR of was targeted by molecular docking with 82 garlic metabolites to identify the most potent QS inhibitors. Five metabolites, quercetin, kaempferol, luteolin, flavone, and rutin, were selected for further evaluation of their impacts on the growth, toxin production, and virulence of .

Novel Essential Oil-Based Thiosemicarbazone Compounds as Potential Fungicides in Controlling Postharvest Diseases of Citrus Fruit.

To develop novel fungicides for controlling postharvest fungal diseases in citrus fruits, 12 essential oil (EO)-based thiosemicarbazones compounds, termed hydrazine-carbothioamide, were prepared according to the condensation method. assays showed that compound 13j exhibited the strongest antifungal activity (minimum inhibitory concentration [MIC] = minimum fungicidal concentration [MFC] = 0.0125 mg/mL) against .

Inhibitory Mechanisms of -2-Hexenal on the Growth of -.

- (-) is one of the most important postharvest pathogens leading to a postharvest loss of citrus by causing sour rot. In this study, the antifungal activity of -2-hexenal, a natural component of essential oil, against - was evaluated. -2-hexenal treatment inhibited the mycelia growth of - with a minimum inhibitory concentration and minimum fungicidal concentration of -2-hexenal at 0.

γ-Cyclodextrin encapsulated thymol for citrus preservation and its possible mechanism against Penicillium digitatum.

The volatility of essential oils greatly limits their industrial applications. Here, we successfully prepared γ-cyclodextrin (γ-CD) inclusion compounds (γ-CDTL) containing thymol (TL) for the control of green mold caused by Penicillium digitatum (P. digitatum) in citrus fruit.

Integrated transcriptomic-metabolomic analysis reveals that cinnamaldehyde exposure positively regulates the phenylpropanoid pathway in postharvest Satsuma mandarin (Citrus unshiu).

Previously, wax + cinnamaldehyde (WCA) was proven to be able to effectively alleviate fruit decay and induce resistance in harvested Satsuma mandarin (Citrus unshiu). However, the potential molecular mechanism is largely unknown. In the present study, transcriptomics, metabolomics and biochemical analyses were combined to clarify this process.

-Cyclodextrin-Encapsulated Cinnamaldehyde for Citrus Preservation and Its Potential Mechanisms against .

In this study, a -cyclodextrin-cinnamaldehyde inclusion compound (-CDCL) was prepared to control green mold caused by () in citrus. The results showed that the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of -CDCL against the mycelial growth of were 2.0 g L and 4.

Exogenous Application of Melatonin to Green Horn Pepper Fruit Reduces Chilling Injury during Postharvest Cold Storage by Regulating Enzymatic Activities in the Antioxidant System.

Chilling injury (CI) caused by exposure to low temperatures is a serious problem in the postharvest cold storage of pepper fruit. Melatonin (MT) has been reported to minimize CI in several plants. To evaluate the effectiveness of MT to minimize CI in green horn pepper and the possible mechanism involved, freshly picked green horn peppers were treated with MT solution at 100 μmol L or water and then stored at 4 °C for 25 d.

A combination of cinnamaldehyde and citral greatly alleviates postharvest occurrence of sour rot in citrus fruits without compromising the fruit quality.

One of the most troublesome postharvest diseases of citrus fruits is sour rot, caused by . Sour rot reduces the shelf life of the fruits leading to massive economic losses. This study investigated the potential for a combination of cinnamaldehyde and citral (CC; 1: 2, v/v) at reducing the incidence of sour rot postharvest and its possible effect on fruit quality.

Cinnamaldehyde promotes the defense response in postharvest citrus fruit inoculated with Penicillium digitatum and Geotrichum citri-aurantii.

Induced resistance in harvested fruit and vegetables is a superior strategy to reduce postharvest decay. In the present study, Cinnamaldehyde (CA) was applied to investigate for its induced resistance against Penicillium digitatum and Geotrichum citri-aurantii. The results showed that 5250 mg CA/L wax was effective concentration in inducing the resistance of citrus fruit to green mold and sour rot.

Citronellal Exerts Its Antifungal Activity by Targeting Ergosterol Biosynthesis in .

Ergosterol (ERG) is a potential target for the development of antifungal agents against , the pathogen of green mold in citrus fruits. This study examined the mechanism by which citronellal, a typical terpenoid of essential oil, acts on ergosterol to exhibit its antifungal activity against . We previously reported that citronellal inhibited the growth of with minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of 1.

Antofine inhibits postharvest green mold due to imazalil-resistant Penicillium digitatum strain Pdw03 by triggering oxidative burst.

The emergence of imazalil (IMZ) resistance in Penicillium digitatum has become a great threat for controlling citrus green mold. In this paper, we investigated the antifungal efficiency and mechanism of an alkaloid antofine against an IMZ-resistant P. digitatum strain Pdw03.

-Anisaldehyde Exerts Its Antifungal Activity Against and by Disrupting the Cell Wall Integrity and Membrane Permeability.

and are the two important postharvest pathogens in citrus, causing about 90% of the total loss of citrus fruit during storage and transportation. Natural fungicides such as essential oils have been widely used instead of chemical fungicides for preventing and controlling postharvest diseases. In this research, -anisaldehyde exhibited a strong inhibitory effect on and , with the minimum inhibitory concentration and minimum fungicidal concentration values of both being 2.

Polyclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for screening of paclobutrazol in fruits.

Paclobutrazol (PBZ) is a plant growth regulator (PGR) widely used in fruit and vegetable cultivation. However, due to the severe toxicity of PBZ, a sub-ppm level maximum residue limit (MRL) was established worldwide. Therefore, it is significant to propose a rapid, sensitive and high throughput screening method for monitoring the PBZ residues in foods.

Cinnamaldehyde Exerts Its Antifungal Activity by Disrupting the Cell Wall Integrity of .

Our previous study showed that cinnamaldehyde (CA) significantly inhibited the mycelial growth of , one of the main postharvest pathogens in citrus fruits. This study investigated the antifungal mechanism of CA against . Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that CA treatment led to clear morphological changes in the cell walls and membranes of .

A Damaged Oxidative Phosphorylation Mechanism Is Involved in the Antifungal Activity of Citral against .

Citral exhibits strong antifungal activity against . In this study, 41 over-expressed and 84 repressed proteins in after 1.0 μL/mL of citral exposure for 30 min were identified by the iTRAQ technique.

Antifungal mechanism of sodium dehydroacetate against Geotrichum citri-aurantii.

This study investigated the potential anti-fungal mechanisms of sodium dehydroacetate (SD) against Geotrichum citri-aurantii. The results showed that the cell wall integrity of G. citri-aurantii was not affected, whereas the membrane permeability of G.

Effect of applying cinnamaldehyde incorporated in wax on green mould decay in citrus fruits.

Background: Green mould caused by Penicillium digitatum is the most damaging postharvest diseases of citrus fruit. Cinnamaldehyde (CA) is a food additive that has potential use in controlling postharvest disease of fruits and vegetables. In this study, the effectiveness of wax with CA (WCA) in controlling Ponkan (Citrus reticulata Blanco) green mould was investigated.

Plasma membrane damage contributes to antifungal activity of citronellal against .

The antifungal activity of citronellal, a typical terpenoid of plant essential oils, against and the possible action mode involved were investigated. Results showed that the mycelial growth and spores' germination of were inhibited by citronellal in a dose-dependent manner. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined to be 1.

Octanal inhibits spore germination of Penicillium digitatum involving membrane peroxidation.

Octanal is a potential alternative to chemical fungicides in controlling postharvest disease of citrus fruit. In this study, the antifungal activity and the underlying mechanism of octanal against spore germination of Penicillium digitatum, one of the main postharvest pathogens in citrus, were investigated. Results showed that octanal at different concentrations (0, 0.

Transcriptional profiling analysis of Penicillium digitatum, the causal agent of citrus green mold, unravels an inhibited ergosterol biosynthesis pathway in response to citral.

Background: Green mold caused by Penicillium digitatum is the most damaging postharvest diseases of citrus fruit. Previously, we have observed that citral dose-dependently inhibited the mycelial growth of P. digitatum, with the minimum inhibitory concentration (MIC) of 1.

Citral exerts its antifungal activity against Penicillium digitatum by affecting the mitochondrial morphology and function.

This work investigated the effect of citral on the mitochondrial morphology and function of Penicillium digitatum. Citral at concentrations of 2.0 or 4.