Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
September 2003
Background: To isolate and identify pathogen of atypical pneumonia in Guangdong.
Methods: Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells, and identified with serological and molecular methods.
Results: A novel coronavirus was isolated from patients with atypical pneumonia, from which an RNA fragment of 279 nt was amplified by nested RT-PCR.