[Study on Application of EMA-PCR Rapid Identification of Infectious and Non-infectious Adenovirus].

Objectives: To investigate the application of cytopathic effect (EMA)-PCR to rapid identification of infectious and non-infectious adenovirus.

Methods: The different dilutions of virus were inoculated to well-grown monolayer cells and then calculated the concentration of virus through the cytopathic effect (CPE). The adenovirus and New Castle disease virus were prepared in a fixed concentration of 10 PFU/mL then diluted in ten-time gradient and the DNA of virus were extracted and PCR were applied, then the target DNA fragments were observed through gel electrophoresis.