Impact of non-emergency surgical timing on postoperative recovery quality in mild or asymptomatic SARS-CoV-2 infected patients: a grouped cohort study.

Objective: To explore the relationship between the timing of non-emergency surgery in mild or asymptomatic SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) infected individuals and the quality of postoperative recovery from the time of confirmed infection to the day of surgery.

Methods: We retrospectively reviewed the medical records of 300 cases of mild or asymptomatic SARS-CoV-2 infected patients undergoing elective general anaesthesia surgery at Yijishan Hospital between January 9, 2023, and February 17, 2023. Based on the time from confirmed SARS-CoV-2 infection to the day of surgery, patients were divided into four groups: ≤2 weeks (Group A), 2-4 weeks (Group B), 4-6 weeks (Group C), and 6-8 weeks (Group D).

Thermo-sensitivity of hybrid nanogels for specific endogenous hydrogen sulfide detection and efficient flash chill treatment.

Thermo-responsive nanogels, poly(NIPAM-AAM) were prepared by a facile method of free radical one-pot precipitation based on monomeric N-isopropylacrylamide (NIPAM). At the same time, surface carboxyl group-modified fluorescent conjugated polymer nanoparticles (PNPs-COOH) were immobilized in the nanogel networks by hydrogen bonding to show bright green photoluminescence and outstanding thermo-responsive properties with a typical two-phase Tai Chi structure. Poly(NIPAM-AAm)-PNPs-COOH displays a larger change of hydrodynamic diameter (Dh) and a reversible volume transition from ∼150 nm at 40 °C to ∼1.

Adaptive immune responses mediated age-related Plasmodium yoelii 17XL and 17XNL infections in 4 and 8-week-old BALB/c mice.

Backgroud: It is important to expound the opposite clinical outcomes between children and adulthood for eradicate malaria. There remains unknown about the correlation between adaptive immune response and age-related in malaria.

Methods: 4 and 8-week-old mice were used to mimic children and adulthood, respectively.

The sensitive "Turn-on" fluorescence platform of ascorbic acid based on conjugated polymer nanoparticles.

Ascorbic acid (AA) is very important for maintaining the normal physiological processes of the organism. In order to overcome the shortcomings of existing methods, such as lower sensitivity, complex operations and expensive instruments, it is urgent to develop a simple and rapid method for the determination of AA in the field of food and pharmaceutical preparations. Conjugated polymer nanoparticles (CPNs) have lately aroused wide concerns because of excellent optical properties, biocompatibility and stability.

Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer.

The calcium ion (Ca) isa highly versatile intracellular signal messenger regulating many different cellular functions. It is important to design probes with good fluorescence and two-photon (TP) active cross-sections (Φ) to explore the concentration distribution of Ca. In this manuscript, a novel TP fluorescence calcium probe (BAPTAVP) with positive charges, based on the classical Ca indicator of BAPTA (1,2-bis(2-aminophenoxy)-ethane--tetra acetic acid), and a conjugated polymer (PCBMB) with negative charges were designed and synthesized.

Highly Sensitive Detection of Melamine Based on the Fluorescence Resonance Energy Transfer between Conjugated Polymer Nanoparticles and Gold Nanoparticles.

Adding melamine as additives in food products will lead to many diseases and even death. However, the present techniques of melamine detection require time-consuming steps, complicated procedures and expensive analytical apparatus. The fluorescent assay method was facile and highly sensitive.

Inhibition of platelet-derived growth factor receptor β reduces reactive glia and scar formation after traumatic brain injury in mice.

Brain injury leads to complex cellular and molecular interactions within the central nervous system. As the glial scar was a mechanical barrier to regeneration, inhibitory molecules in the forming scar and methods to overcome them have suggested molecular modification strategies to allow neuronal growth and functional regeneration. Here we investigated the roles of PDGFRβ signaling in regulating astrocyte reactivity and scar formation in mice following traumatic brain injury (TBI).

[Construction of recombinant shuttle plasmid pIMP1-eHER2/neu and screening and identification of its stable Clostridium sporogenes transformants].

Aim: To construct recombinant clostridium sporogenes modified with the extracellular domain of human oncogene HER2/neu, to lay a foundation for further study of its antitumor effect.

Methods: The extracellular domain (ECD) of HER2/neu gene was attached to the downstream of promoter and signal sequence of clostridia endo-1, 4-glucanase (eglAp) by SOE-PCR to construct fusion gene eglAp-HER2/neu, which was then inserted into E.coli-clostridia shuttle plasmid pIMP1 to construct recombinant plasmid pIMP1-eHER2/neu.

[Knocking down human CD59 gene expression decreased protection to complement-mediated cytolysis].

Aim: To construct recombinant vectors expressing siRNA that target CD59 gene and a stable-inhibit cell line A2780 in order to analyze the role of CD59 in the protection to complement-mediated cytolysis.

Methods: The 60 bp encoded targeting CD59 gene shRNA sequence was cloned into pSUPER vector with DNA recombinant technique. The ovary cancer cell A2780 was transfected with this recombinant plasmid using liposome and the stable strains was selected by using G418-medium, CD59 mRNA and protein level was detected by RT-PCR and Western blot, and its function was analysed by dye release assay.