Integrated transcriptomic analysis reveals hub genes involved in diagnosis and prognosis of pancreatic cancer.

Background: The hunt for the molecular markers with specificity and sensitivity has been a hot area for the tumor treatment. Due to the poor diagnosis and prognosis of pancreatic cancer (PC), the excision rate is often low, which makes it more urgent to find the ideal tumor markers.

Methods: Robust Rank Aggreg (RRA) methods was firstly applied to identify the differentially expressed genes (DEGs) between PC tissues and normal tissues from GSE28735, GSE15471, GSE16515, and GSE101448.

Robust analysis of novel mRNA-lncRNA cross talk based on ceRNA hypothesis uncovers carcinogenic mechanism and promotes diagnostic accuracy in esophageal cancer.

Background: ceRNAs have emerged as pivotal players in the regulation of gene expression and play a crucial role in the physiology and development of various cancers. Nevertheless, the function and underlying mechanisms of ceRNAs in esophageal cancer (EC) are still largely unknown.

Methods: In this study, profiles of DEmRNAs, DElncRNAs, and DEmiRNAs between normal and EC tumor tissue samples were obtained from the Cancer Genome Atlas database using the DESeq package in R by setting the adjusted <0.

Label-free microRNA detection based on terbium and duplex-specific nuclease assisted target recycling.

In this paper, we describe a novel label-free fluorescence method for microRNA-21 (miR-21) detection based on terbium (Tb(3+)) and duplex-specific nuclease (DSN) assisted target recycling. Capture probes (Cps), containing a target-binding part and a signal-output part, are immobilized on magnetic beads (MBs). In the presence of the target miR-21, it hybridizes with the target-binding part of a Cp to form a DNA-RNA heteroduplex.

[Clinical characteristics of 32 cases of tsutsugamushi disease in Suqian Municipality].

Objective: To understand the clinical characteristics of tsutsugamushi disease in Suqian Municipality, Jiangsu Province so as to guide the diagnosis and treatment of this disease.

Methods: The clinical data of 32 patients diagnosed as tsutsugamushi disease in our hospital during the past 2 years were analyzed retrospectively.

Results: Tsutsugamushi disease occurred frequently between August and December.

Effects of nitrate supply site on selenite uptake by rice roots.

Selenite uptake by rice roots is regulated by many factors. The goal of this paper was to study the effects of the nitrate supply site on selenite uptake by rice roots. Using the excised-root experiment system, we found that, due to anion-anion interactions, nitrate can partly block the selenite uptake by rice roots by local action (a corporate supply of nitrate and selenite in the same portion).