[The Diagnostic Value of Ret-He in Predicting Latent Iron Deficiency in Female Blood Donors].

Objective: To explore the application value of reticulocyte hemoglobin equivalent (Ret-He) for diagnosing latent iron deficiency in female plateletpheresis donors.

Methods: A total of 230 female plateletpheresis donors in Fujian Blood Center from January to February 2022 were selected as the research group and divided into three groups: normal group, iron depletion (ID) group and iron deficient erythropoiesis (IDE) group, according to the severity of iron deficiency. The level of hemoglobin (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), coefficient of variation of red cell distribution width (RDW-CV) and Ret-He were measured by using the Sysmex XN automated hematology analyzer.

[Short-term nitrogen addition reduces soil microbial nitrogen fixation rate in subtropical and forests].

Biological nitrogen (N) fixation is an important source of N in terrestrial ecosystems, but the response of soil microbial N fixation rate to N deposition in different forest ecosystems still remains uncertain. We conducted a field N addition experiment to simulate atmosphere N deposition in subtropical and forests. We set up three levels of nitrogen addition using urea as the N source: 0 (control), 40 (low N), and 80 g N·hm·a(high N) to examine the chemical properties, microbial biomass C, enzyme activities, and gene copies of top soils (0-10 cm).

F-actin/DRP1 axis-mediated mitochondrial fission promotes mitophagy in diabetic submandibular glands.

Background: Diabetes is accompanied by a high prevalence of hyposalivation, causing severe damage to oral and systemic health. Mitochondrial dynamics play important roles in the pathogenesis of various diabetic complications; however, little is known about their roles in diabetic hyposalivation.

Materials And Methods: A diabetic mouse model and a high glucose (HG)-induced diabetic submandibular gland (SMG) cell model were employed.

Membrane-protected magnetic covalent organic framework for efficient extraction of estrogens in dairy products.

The presence of estrogens residues in dairy products is a growing concern due to their potential health risk. Herein, in this study, we have developed a membrane-protected magnetic solid-phase extraction (MP-MSPE) method that utilized a magnetic adsorbent (FeO@COF-LZU1) with in-situ growth for the efficient extraction of estrone (E1), 17β-estradiol (E2), and estriol (E3). When combined with HPLC-FLD, this method allows for the efficient detection of estrogens in dairy products.

[Relationship between Iron Metabolic Parameters and Platelet Counts in Blood Donors].

Objective: To investigate the correlation of iron metabolic parameters with platelet counts in blood donors.

Methods: A total of 400 blood donors who met requirements of apheresis platelet donation were collected, and their hematological parameters were analyzed. The donors were divided into low ferritin group and normal group, the differences of hematological parameters between the two groups were compared, and the correlation of iron metabolic parameters and routine hematology parameters with platelet counts were analyzed.

Enzyme stoichiometry evidence revealed that five years nitrogen addition exacerbated the carbon and phosphorus limitation of soil microorganisms in a forest.

The activity and stoichiometry of soil extracellular enzyme can provide a good indication for changes in soil nutrient availability and microbial demands for nutrients. However, it remains unclear how would nitrogen (N) deposition affect nutrient limitation of microbes in subtropical forest soils. We conducted a 5 years N addition experiment in a subtropical forest.

A Selective TRPC3 Inhibitor Pyr3 Attenuates Myocardial Ischemia/Reperfusion Injury in Mice.

An emerging body of evidence indicates that transient receptor potential TRP channels act as important mediators for a wide variety of physiological functions and are potential targets for drug discovery. Our previous study has identified transient receptor potential channel 3 (TRPC3) and TRPC6 as cation channels through which most of the damaging calcium enters, aggravates pathological changes in vivo and increases ischemia/reperfusion (I/R) injury in mice. This study aimed to verify the effects of TRPC3 inhibitor Pyr3 on myocardial I/R injury in mice.

Tannins from senescent Rhizophora mangle mangrove leaves have a distinctive effect on prokaryotic and eukaryotic communities in a Distichlis spicata salt marsh soil.

Due to climate warming, tannin-rich Rhizophora mangle migrates into tannin-poor salt marshes, where the tannins interfere with the biogeochemistry in the soil. Changes in biogeochemistry are likely associated with changes in microbial communities. This was studied in microcosms filled with salt marsh soil and amended with leaf powder, crude condensed tannins, purified condensed tannins (PCT), all from senescent R.

[Responses of soil phosphorus fractions and microorganisms to nitrogen application in a subtropical Phyllostachys pubescen forest].

Phosphorus (P) is an important nutrient for plant and microbial growth. Soil P availabi-lity is poor in subtropical areas. Long-term heavy nitrogen (N) deposition might further reduce P availability.

Nuclear FAM289-Galectin-1 interaction controls FAM289-mediated tumor promotion in malignant glioma.

Background: FAM92A1-289(abbreviated FAM289) is recognized as one of the newly-discovered putative oncogenes. However, its role and molecular mechanisms in promoting cancer progression has not yet been elucidated. This study was performed to reveal its oncogenic functions and molecular mechanisms in human glioblastoma multiforme (GBM) cell models with knockdown or overexpression of FAM289 in vitro and in vivo.

[Effects of nitrogen deposition on the concentration and spectral characteristics of dissolved organic matter in soil in Moso bamboo plantations.].

The subtropical zone in China is one of the regions most affected by nitrogen deposition. Soil dissolved organic matter (DOM) is considered to be an important indicator of soil organic matter. Nitrogen deposition may alter the quality and quantity of soil DOM by changing soil microbial activity.

[Nutrient and metabolic responses of the leaves of Cunninghamia lanceolata seedlings to warming and reduced precipitation in different season].

We examined the effects of warming (+5 ℃) and reduced natural precipitation (-50%) on nutrient status and physiological indices of Cunninghamia lanceolata seedlings during winter and summer in subtropical China. The results showed that seasonal changes in temperature and precipitation caused the seasonal differences in plant nutrient contents and metabolites levels. Contents of carbon, nitrogen, phosphorus, and potassium in leaves in winter were significantly higher than those in summer.

[Effects of precipitation reduction on the composition and stability of soil organic matter in a young Cunninghamia lanceolata plantation.].

It is hard to predict the response of soil organic matter (SOM) to global climate change due to its heterogenous chemical structure. With the development of molecular techniques to identify the structure, sources and stages of SOM degradation, long-standing questions regarding the composition and stability of SOM might be resolved. To investigate the effects of changes in precipitation patterns on the stability of SOM, we analyzed the specific compositions and extent of degradation of SOM using biomarkers, in a young Cunninghamia lanceolata plantation after reducing 50% of precipitation (P) for two years.

Biatractylolide Modulates PI3K-Akt-GSK3-Dependent Pathways to Protect against Glutamate-Induced Cell Damage in PC12 and SH-SY5Y Cells.

Biatractylolide, isolated from the ethyl acetate extract of , has shown various pharmacological activities such as antitumor and antioxidant activities. In this work, we aim to study the protective effect of biatractylolide on glutamate-induced rat adrenal pheochromocytoma cell (PC12) and human bone marrow neuroblastoma cell line (SH-SY5Y) injury and preliminarily explore its mechanism. The results showed that glutamate was cytotoxic with an inhibitory concentration 50% (IC50) of 8.

Functional investigation on aromatase in endometrial hyperplasia in polycystic ovary syndrome cases.

Objective: To explore the possible significance of aromatase P450 in endometrial hyperplasia with a background of polycystic ovary syndrome (PCOS).

Methods: Immunohistochemistry was used to determine the expression of aromatase P450 in endometrium of PCOS patients. Semiquantitative analysis of aromatase P450 expression of mRNA and protein level wasalso carried out by real-time quantitative RT-PCR method.

Combination of serum inhibin B and follicle-stimulating hormone levels can not improve the diagnostic accuracy on testicular sperm extraction outcomes in Chinese non-obstructive azoospermic men.

Background: It is still controversial whether the serum inhibin B level is a superior predictor of the presence of sperm in testicular sperm extraction (TESE) in azoospermic men compared with serum follicle-stimulating hormone (FSH). In this study, we evaluated the diagnostic accuracy of serum inhibin B levels as a predictor of the outcome of TESE in Chinese non-obstructive azoospermic men and compared it with the traditional marker serum FSH and testicular volumes.

Methods: Basal values of serum hormone levels, testicular volumes and histological evaluation of 305 Chinese non-obstructive azoospermic men were analyzed.

Activation of epidermal growth factor receptor mediates reperfusion arrhythmias in anaesthetized rats.

Aims: Epidermal growth factor receptor (EGFR) plays a critical role in the development and function of the heart. Previous studies have demonstrated that EGFR is involved in regulating electrical excitability of the heart. The present study was designed to investigate whether EGFR activation would mediate cardiac arrhythmias induced by reperfusion in anaesthetized rats.

Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury.

Background: It remains almost a helpless situation for the recurrent implantation failure and pregnancy loss caused by endometrial injury at present. The purpose of this study was to develop a rabbit model of endometrial mechanical injury that could provide a research platform for this difficult clinical predicament.

Methods: Three experiments were conducted.

Alteration of ERβ gene RsaI polymorphism may contribute to reduced fertilization rate and embryonic developmental competence.

This paper aims to determine the possible role of estrogen receptor-β (ERβ) gene RsaI polymorphism on sperm fertility and early embryonic development in humans. Three groups of Chinese men were recruited: in vitro fertilization (IVF) group, including 374 couples who underwent conventional IVF; intracytoplasmic sperm injection (ICSI) group, including 294 couples who underwent an ICSI procedure using ejaculated sperm; and azoospermic group, consisting of 197 couples who underwent ICSI using either testis or epididymis sperm. RsaI polymorphism in the ERβ gene was detected by polymerase chain reaction (PCR)-restriction fragment length polymorphism technique; fertilization and high-quality embryo rates were evaluated for each group.

[Study of HBV-DNA in ovarian granulosa cells of hepatitis B carriers].

Objective: To study the infection status of hepatitis B virus in ovarian granulosa cells of female hepatitis B carriers.

Methods: The follicular fluid of 6 female carriers of hepatitis B (HBsAg+, HBeAg+, HBcAb+) was used to extract granule cells and prepare smears. The samples were detected via in situ hybridization by digoxin-labeled HBV-DNA probe.

Characteristics of abnormal menstrual cycle and polycystic ovary syndrome in community and hospital populations.

Background: Polycystic ovary syndrome (PCOS) is considered to be the most common endocrine disorder in women of reproductive age. The involvement of an abnormal menstrual cycle in the etiology of PCOS remains unclear. We aimed to analyze the characteristics of abnormal menstrual cycles and their association with PCOS in community and hospital patient populations.

[Outcome of intracytoplasmic sperm injection cycle: fresh compared to cryopreserved-thawed testicular and epididymal spermatozoa.].

Objective: To compare clinical outcome of intracytoplasmic sperm injection (ICSI) cycle by using fresh and cryopreserved-thawed testicular and epididymal spermatozoa in azoospermic patients.

Methods: Between September 2006 and May 2007, 208 azoospermic patients underwent in vitro fertilization (IVF) were treated in Center of Reproductive Medicine, Peking University Third Hospital. Those couples were divided into two groups based on their wishes, including 171 cases in fresh group and 37 cases in cryopreserved-thawed group.

Inhibition of tetramethylpyrazine on P-gp, MRP2, MRP3 and MRP5 in multidrug resistant human hepatocellular carcinoma cells.

Some membrane transporters in liver, such as P-glycoprotein, multidrug resistance-associated protein 2 (MRP2), MRP3, and MRP5 can lead to a complex multidrug resistance (MDR) to antineoplastic agents. How to inhibit these proteins is still an issue. Tetramethylpyrazine is a bioactive constituent isolated from the root of Ligusticum chuanxiong Hort, a Chinese herb.

[Acupuncture intervention combined with assisted reproductive technology: its different effects at different time points during the in vitro fertilization-embryo transfer course].

Recently the combination of acupuncture with assisted reproductive technology (ART) to increase the outcomes of ART is being widely studied. In this article, the literatures concerning random controlled clinical trials since 2002 are reviewed and the designs of the trials, especially the timing of acupuncture, are evaluated. Over the past 5 years, the related clinical trials have primarily showed that acupuncture done immediately before and after embryo transfer might increase the assisted reproduction rates, but still requiring further high quality trials with large samples; in addition, different stimulation modes could produce different result, and so far there has not been a consensus as to the optimal time-point for the acupuncture intervention during the in vitro fertilization-embryo transfer (IVF-ET) course.