An electrochemical enzymatic nanoreactor based on dendritic mesoporous silica nanoparticles for living cell HO detection.

The selective and quantitative detection of cellular H2O2 is essential for understanding its roles in physiology and pathology. A new electrochemical H2O2 biosensor, fabricated by immobilizing horseradish peroxidase onto dendritic mesoporous silica nanoparticles (HRP/DMSNs), is employed for living cell H2O2 detection. Taking advantage of the large pore volume and highly accessible internal surface areas of DMSNs, HRP/DMSNs display higher enzymatic loading, better stability and bioactivity in comparison with HRP on nonporous silica nanoparticles (NSNs).

Polydopamine functionalized nanoporous graphene foam as nanoreactor for efficient electrode-driven metabolism of steroid hormones.

Understanding the enzymatic reaction kinetics of cytochrome P450 (CYP) within confined spaces is essential for the development of efficient CYP bioreactors in vitro. Herein, a facile electrochemical enzymatic nanoreactor (CYP3A4/PNGFs) is constructed by immobilizing CYP3A4 inside polydopamine (PDA) modified nanoporous graphene foams (PNGFs) whose pore diameter is controllable by the template of silica spheres. Taking advantages of the unique 3-D structure of NGFs and the ideal crosslinking agent of PDA, CYP3A4 is easily absorbed into PNGFs with high stability and bioactivity.