Authenticatin Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay.

6-Benzylaminopurine (6-BA), a plant growth regulator with cytokinin-like properties, was recently reported to be illegally used in bean sprouts to increase their commercial appearance. It is still nevertheless challenging to quickly detect this adulteration. In this work, four novel haptens (haptens 1-4) of 6-BA were rationally designed with computer-assisted modeling analysis and then synthesized for use as immunizing haptens to produce antibodies.

Identifying fungicide difenoconazole as illegal growth regulator in vegetable: Computer-aided hapten similarity to enhance immunoassay sensitivity.

Difenoconazole, a fungicide with broad-spectrum properties, has recently been found to have been used illegally used as a plant growth regulator in Brassica campestris, with the intent of inducing thick stems and dark green leaves. However, analysts have encountered challenges in implementing a rapid surveillance screening approach for this purpose. In this study, a novel hapten was designed to improve the analytical performance of difenoconazole immunoassay.

Developing an ultrasensitive immunochromatographic assay for authentication of an emergent fraud aminopyrine in herbal tea.

Aminopyrine is a nonsteroidal anti-inflammatory drug only for medical purposes, however, it has been illegally added in traditional Chinese herbal teas for fraud activity recently. In this study, a specific antibody against aminopyrine with IC of 3.00 ng/mL was obtained for the first time by a rational hapten design.

A sensitive and quantitative immunochromatographic assay for simultaneous detection of three stimulant laxatives in slimming food.

Bisacodyl, sodium picosulfate and their metabolite bis-(p-hydroxyphenyl)-pyridyl-2-methane (BHPM) are clinically used to treat constipation. In this study, with the rational hapten design, a broad-specific and highly sensitive monoclonal antibody (mAb) was obtained with half of inhibitory concentrations of 0.16, 0.

Ultrasensitive Magnetic Assisted Lateral Flow Immunoassay Based on Chiral Monoclonal Antibody against -(-)-Salbutamol of Broad-Specificity for 38 β-Agonists Detection in Swine Urine and Pork.

Screening for ″zero tolerance″ β-agonists requires broad-specificity and sensitivity methods. Herein, -(-)-salbutamol (SAL) is chirally separated and designed as a hapten, and a monoclonal antibody (mAb) was first prepared with an IC of 0.27 ng/mL, which can recognize 38 β-agonists simultaneously.