Development of recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid and simple detection of Tylenchulus semipenetrans in soil.

Background: Tylenchulus semipenetrans, the causal agent of citrus slow decline disease, is one of the most destructive plant-parasitic nematodes in all citrus-growing regions of the world, causing significant reductions in citrus growth and yield. Accurate and rapid detection of T. semipenetrans is critical for the diagnosis and effective control of the disease.